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Measure Stress in Mice with as little as 1 µL of Serum

 

  • As Little as 1 μL Serum or Plasma Sample Required for Duplicate Measurements
  • Proven Results  – Over 200 Literature Citations for EIA and CLIA
  • No extractions necessary
  • Extended Range – 10,000 to 78 pg/ml (EIA) or 3000 to 12.3 pg/ml (CLIA)
  • Results in 90 minutes (EIA) or 2 hours (CLIA)
  • Multispecies – Validated in mice, rats, monkeys, other mammals, birds, reptiles, amphibians
  • High quality, Reliable and Economical
  • Superior stability at 4˚C

Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland in response to stimulation by adrenocortocotropic hormone. Glucocorticoids such as corticosterone guide fundamental processes associated with converting sugar, fat, and protein stores to usable energy; inhibiting swelling and inflammation, and suppressing immune response following a stress event.

Most assays for corticosterone require solvent extraction steps to measure serum or plasma corticosterone levels.  However, these extraction protocols typically require relatively large volumes of serum or plasma making them problematic with smaller samples such as mouse tail bleeds.

Our Corticosterone EIA (K014-H) and CLIA (K014-C) kits overcome this serum and plasma volume problem by allowing the direct measurement of corticosterone from as little as 1μL of mouse serum or plasma. Serum or plasma samples are treated with an equal volume of Arbor Assays’ Dissociation Reagent that liberates the corticosterone from any binding proteins. The mixture is then diluted 1:50 or greater with assay buffer and added directly to the well of the provided microtiter plate for analysis by EIA/CLIA without any extractions.

The EIA provides results in 90 minutes and the chemiluminescent immunoassay (CLIA) in 2 hours. The EIA standard range spans 10,000 to 78 pg/mL and the CLIA has a range from 3,000 to 12.3 pg/mL with a measured sensitivity of 6.7 pg/mL. The CLIA allows concentrations of <0.004 μM of corticosterone to be measured. Typical c57bl/6j mouse serum corticosterone levels fall at 0.08 ± 0.01 μM and therefore can be measured in this assay without extraction.

 

Watch our Mouse Tail Bleed Sample Prep Video for more information

See Related Stress Kits

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