Endothelin-1 (ET-1) EIA Kit

Features

  • Most Sensitive
  • Multi Species
  • USE - Measure ET-1 in Plasma, Serum and Biological Samples
  • SAMPLE - Serum, Plasma, or TCM
  • SAMPLES/KIT - 39 in Duplicate
  • SENSITIVE - Measures < 0.58 pg/mL ET-1
  • STABILITY - All Reagents Stable at 4˚C
  • ECONOMICAL - No C18 Cartridges - Supplied Extraction Solution

The DetectX® Endothelin-1 (ET-1) Immunoassay kit is designed to quantitatively measure ET-1 present in a variety samples and tissue culture media. An ET-1 standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture ET-1 present in the sample. After a 60 minute incubation, the plate is washed and a peroxidase conjugated ET-1 antibody is added. The plate is again incubated for 60 minutes and washed. Substrate is then added to the plate, which reacts with the bound ET-1 conjugated antibody. After a third incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the ET-1 in the sample is then calculated.

K045-H

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