Cortisone Chemiluminescent Immunoassay Kit

Features

  • Multi Species
  • USE - Measure the Activity of 11B-HSD Enzymes in 2 hours
  • SAMPLE - Plasma, Serum, Saliva, Urine, Media, and Fecal Extracts
  • SAMPLES/KIT - 37 or 229 in Duplicate
  • STABILITY - Stable 4˚C Liquid Reagents

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The DetectX® Cortisone Chemiluminescent Immunoassay (CLIA) kit is designed to quantitatively measure Cortisone present in samples. A cortisone standard is provided to generate a standard curve for the assay. After a two hour incubation the plate is washed and the chemiluminescent substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce light. The generated light is detected in a multilabel microtiter plate reader.

Cortisone and cortisol concentrations vary due to the activity of two 11ß-hydroxysteroid dehydrogenases (11-HSD). While most tissues have the ability to express either enzyme, 11ß-HSD1 is found primarily in the liver where it converts cortisone to cortisol while 11ß-HSD2 is found in tissues such as the kidney where cortisol receptor binding is required. 11ß-HSD2 deactivates cortisol to cortisone, prohibiting receptor activation. This glucocorticoid “shuttle” helps to initiate and regulate the anti-inflammatory response. Monitoring the ratio of cortisone:cortisol has applications in diabetes, obesity, metabolic syndrome, osteoporosis, and chronic fatigue syndrome in addition to adrenal diseases. Cortisone and cortisol concentrations exhibit a predictable diurnal pattern and can be measured in extracted dried feces, or in serum, plasma, saliva and urine. A recent publication has suggested that salivary cortisone is a good surrogate marker for serum cortisol.

Cortisone Chemiluminescent Standard Curve

Data Reduction Template by MyAssays

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