- SENSITIVE: Measure < 500 fmol Free Thiol
- RAPID: Bright Fluorescence in Seconds
- SOLUTION STABILITY: 2 Months at 4°C
- LARGE STOKES SHIFT: Excite at 390 nm, Read at 510 nm
- LOW BACKGROUND: Almost No Signal from Unreacted ThioStar®
- VERSATILE: Soluble in Common Solvents and Water
- FLEXIBLE FORMAT: Microplates, Cuvettes or HPLC
Simple, Stable and Sensitive
The ThioStar® molecule is designed to quantitatively measure thiol groups generated or present in samples. The non-fluorescent ThioStar dye covalently binds to any free thiol groups to yield a highly fluorescent product. After mixing the sample with ThioStar and incubating at room temperature the fluorescent product is read at 510 nm with excitation at 390 nm.
NEW DATA: Careful in-house examination of the solution stability of ThioStar® dissolved in dry DMSO has shown that the ThioStar® dye retains 100% of its ability to react with free thiol groups for up to 2 months when stored desiccated at 4°C. Allows more convenient and efficient use of this thiol reactive dye.
Shortly after its introduction, ThioStar® was used to detect free thiol groups in unstimulated CD4+ T cells in Dr. Peter Hoffmann’s lab at the University of Hawaii. Their results are in the paper “Dietary Selenium Modulates Activation and Differentiation of CD4+ T Cells in Mice through a Mechanism Involving Cellular Free Thiols”. Semi Park in Professor Stephen Lippard’s lab at MIT used our Thiol Fluorescent Detection kit, K005-F1 (which uses ThioStar® as the detection reagent), to assess free thiol in recombinant HMGB1 domain A in the paper “Redox State-Dependent Interaction of HMGB1 and Cisplatin-Modified DNA”. In a 2012 paper in Microbial Cell Factories entitled “A novel expression system for production of soluble prion proteins in E. coli”, ThioStar® was used to quantitate free sulfhydryls in soluble and refolded recombinant prion proteins. Additional recent publications are listed on the Thiol product page.
The Arbor Assays DetectX® Thiol Fluorescent Detection Kit offers a supersensitive method to measure free thiol groups in a variety of samples using ThioStar®. Supplied with all components needed to measure 39 samples in duplicate utilizing 4°C stable reagents. Can be used for protein samples treated with guanidine hydrochloride solutions. Sensitivity is less than 5 nM.
Typical Thiol Fluorescent Kit Data