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The DetectX® Allopregnanolone Immunoassay (EIA/ELISA) kit is designed to quantitatively measure Allopregnanolone present in serum, plasma, extracted dried fecal samples, urine and tissue culture media samples.
An allopregnanolone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An allopregnanolone-peroxidase conjugate is added to the wells. The binding reaction for the EIA/ELISA is initiated by the addition of a polyclonal antibody to allopregnanolone to each well. After incubation the plate is washed and substrate is added. The substrate reacts with the bound allopregnanolone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color from the EIA/ELISA is detected in a microtiter plate reader capable of measuring 450nm wavelength.
Allopregnanolone aids neurogenesis and has been found to reverse neuron proliferative deficit and cognitive deficits in a mouse model of Alzheimer’s disease. Allopregnanolone has also been shown to restore functionality in a mouse model of Parkinson’s Disease. It has also been shown to improve behavioral problems in post-traumatic stress disorder.