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Cyclic Nucleotide Kits

2′,3′-Cyclic GAMP Direct EIA Kit
The innate immune system is the body’s crucial first defense against viral infections and it is dependent upon a group of pattern recognition receptors. cGMP-AMP Synthase (cGAS) responds to the presence of viral DNA in the cytosol by producing 2’,3’ cGAMP. Cyclic GAMP in turn binds to STING leading to TBK1-mediated IRF3 activation and the production of type 1 IFN. Understanding the STING pathway and the role cGAMP plays in innate response to viral infection is of great interest as a possible mechanism for pharmaceutical intervention to treat cancer, lupus and infections.

2′,3′-Cyclic GAMP (cGAMP) Direct EIA Kits, K067-H1/H5
FEATURES:

  • Use – Measure cGAMP in STING signaling in tissues and cells
  • Range – 50 pmol/mL – 0.205 pmol/mL
  • Time to Answer – 2.5 hours
  • Sensitivity – Measure 4 fmol per sample
  • Samples/Kit – 39 or 231 in duplicate
  • Stability – Liquid, 4°C Stable Reagents
  • Readout – Colorimetric, 450 nm

Use code: NUCLEOTIDE100 to save $100 on your order


Cyclic AMP (cAMP) Kits
Cyclic AMP (cAMP) is one of the most important second messengers and a key intracellular regulator. Discovered by Sutherland and Rall in 1957, it functions as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Cyclic AMP is produced by the enzyme adenylate cyclase, and the enzyme is activated by the hormones glucagon and adrenaline and by G protein. cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterase. Other biological actions of cAMP include regulation of innate immune functioning, axon regeneration, cancer, and inflammation.

Cyclic AMP (cAMP) Direct EIA Kits, K019-H1/H5Citations
FEATURES:

  • Use – Measure cAMP in 2.5 Hours
  • Sample – Lysates, Urine, Plasma, Saliva, Tissue
  • Samples/Kit – 39 or 231 in Duplicate
  • Sensitivity – Measure < 5 fmol cAMP in sample
  • Stability – Stable 4˚C Liquid Reagents
  • Readout – Colorimetric, 450 nm

Cyclic AMP (cAMP) Direct CLIA Kits, K019-C1/C5Citations
FEATURES:

  • Use – Measure cAMP in 2 hours
  • Sample – Lysates, Urine, Plasma, Saliva, Tissue
  • Samples/Kit – 38 or 230 in Duplicate
  • Sensitivity – Measure < 1 fmol cAMP per sample
  • Stability – Stable 4˚C Liquid Reagents
  • Readout – Glow Luminescence

Use code: NUCLEOTIDE100 to save $100 on your order


Cyclic GMP (cGMP) Kits
Cyclic GMP is a critical and multifunctional second messenger present at levels typically 10-100 fold lower than cAMP in most tissues. Intracellular cGMP is formed by the action of the enzyme guanylate cyclase on GTP and degraded through phosphodiesterase hydrolysis. Guanylate cyclases (GC) are either soluble or membrane bound. Soluble GCs are nitric oxide responsive, whereas membrane bound GCs respond to hormones such as acetylcholine, insulin and oxytocin. Other chemicals like serotonin and histamine also cause an increase in cGMP levels. Cyclic GMP regulates cellular composition through cGMP-dependent kinase, cGMP-dependent ion channels or transporters, and through its hydrolytic degradation by phosphodiesterase.

Cyclic GMP (cGMP) Direct EIA Kits, K020-H1/H5Citations
FEATURES:

  • Use – Lyse, Stabilize, Measure cGMP in one step
  • Sample – Lysate, Urine, Plasma, Saliva and Tissue
  • Samples/Kit – 39 or 231 in Duplicate
  • Sensitivity – Measure less than 10 fmol cGMP per sample
  • Stability – Stable 4˚C Liquid Reagents
  • Readout – Colorimetric, 450 nm

Cyclic GMP (cGMP) Direct EIA Improved Sensitivity Kits, K065-H1/H5Citations
Why Another Kit? Cyclic GMP levels are generally present at a full order of magnitude lower than those found for cyclic AMP. These differences can complicate efforts to measure cGMP in certain sample types. To overcome this obstacle, we’ve developed this improved sensitivity cyclic GMP assay to meet our goal of providing the easiest, most sensitive assays available for cyclic GMP. The improved sensitivity cyclic GMP assay is 2-fold more sensitive than current assays on the market and measures cGMP with femtomole sensitivity.

FEATURES:

  • Use – Lyse, Stop PDE Activity and Stabilize in One Step
  • Sensitivity – 5 fmol per sample, 2-fold more sensitive then K020-H cGMP kits
  • Sample – Measure cGMP in Cells, Plasma, Urine, Saliva & Tissue from Any Species
  • Samples/Kit – 38 or 230 in Duplicate
  • Consistent – Appropriate for long-term studies
  • Readout – Colorimetric, 450 nm

Cyclic GMP (cGMP) Direct CLIA Kits, K020-C1/C5Citations
FEATURES:

  • Use – Lyse, Stabilize, Measure cGMP in one step
  • Sample – Lysate, Urine, Plasma, Saliva and Tissue
  • Samples/Kit – 39 or 231 in Duplicate
  • Sensitivity – Measures as Little as 1 fmol per sample
  • Stability – Stable 4˚C Liquid Reagents
  • Readout – Glow Luminescence

Use code: NUCLEOTIDE100 to save $100 on your order

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