…in the body are regulated by the activity of the two 11β-hydroxysteroid dehydrogenase (11β-HSD) enzymes. 11β-HSD1, predominantly found in the liver, converts cortisone to cortisol, while 11β-HSD2, located in tissues…
…dehydrogenase (11β-HSD) enzymes regulate the balance between cortisol and cortisone. 11β-HSD1, mainly found in the liver, converts cortisone to cortisol, whereas 11β-HSD2, present in kidney tissues, is vital for cortisol…
…the Epiandrosterone concentration based on the standard curve. Background: Epiandrosterone, a metabolite of dehydroepiandrosterone (DHEA), is primarily produced in peripheral tissues through the action of the enzyme 5α-reductase. This steroid…
…from androstenedione originating from the gonads or adrenal cortex, and also from estradiol by 17-hydroxysteroid dehydrogenase. Additionally, the aromatase (CYP19) enzyme converts androstenedione into estrone. Estrone plays a significant role…
…understanding galactose metabolism and identifying metabolic disorders like galactosemia. Galactosemia arises from an enzyme deficiency in the Leloir pathway or excessive galactose intake. Accurate monitoring of galactose levels is also…
…nervous, and immunological systems, primarily through guanylate cyclase activation. NO is bio-available through endogenous generation by NOS enzymes and through ingestion as nitrates or nitrites, which are subsequently converted into…
…acid via the cyclooxygenase (COX) enzymes. COX-1 and COX-2 convert arachidonic acid into PGH2, which is then processed into PGE2 and other prostanoids. PGE2 is known for its involvement in…
…when cyclic AMP (cAMP) levels rise following stimulation of G Protein-coupled receptors and adenylyl cyclase. The expressed PKA holoenzyme comprises two catalytic (C) and two regulatory (R) subunits. In its…
…the field of pharmaceutical research, understanding thiol dynamics is important for drug development. This is particularly true for medications targeting enzymes or proteins with critical thiol groups. This kit’s precision…
…curve. Background: Alkaline Phosphatase (ALP) is an enzyme that circulates in the bloodstream and plays a pivotal role in de-phosphorylating various proteins across the body. Present in higher order organisms,…
…free enzyme (Ki=0.4 nM and Ki´=0.3 nM, respectively). NAD+ depletion by FK-866 directs delayed cell death by apoptosis in Hep-G2 human liver carcinoma cells (IC50=~1 nM). Causes premature senescence in…