2′,3′-cGAMP STING-Based FRET Detection Kit

Features

  • Multi Species
  • Use - Detection of 2',3'-cGAMP
  • Time to Answer - 15 minutes
  • Sample - Cell Lysates, Tissue Extracts, Tissue Culture Media
  • Sensitivity - 82.02 pmol/mL
  • Standard Range - 500 - 131 nM
  • Sample/Kits - up to 40 or 232 in duplicate
  • Readout - Fluorescence: Excitation 458 nm, Emission: 600 nm/490 nm

STING was used as the base for a biosensor developed by Pollock et al. for in vivo and in vitro testing. Murine STING was chosen as it has demonstrated a higher propensity to bind to bacterial and eukaryotic CDN’s versus human STING, lending to potentially broader application. This STING biosensor (BioSTING) is a fluorescence resonance energy transfer (FRET) based sensor designed to detect 2’,3’-cGAMP in real-time within living cells. FRET is a fluorescence detection platform that is based on the distance dependent relationship between two fluorophores. In the case of BioSTING, mTFP and mKO2 are the fluorophores of choice. When mTFP is excited with a wavelength of 458 nm, the emission is detected at 490 nm when no ligand (2’,3’-cGAMP) is present. When BioSTING binds to the ligand, the two fluorophores are positioned in closer proximity to each other, allowing the fluorescence to be transferred from mTFP to mKO2, changing the emission wavelength to 600 nm. Using this detection method, BioSTING shows applicability for in vitro high-throughput screening for CDN production modulation and direct screening for STING agonists and antagonists.

 

Standard Curve

 

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