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- Assay Type Detection Kit
- Sample Types Serum, Plasma (EDTA and Heparin), Cell Lysates, WB, RBCs, Urine, Tissue
- Sensitivity 45 nM (Free GSH), 48 nM (Total GSH)
- Species GSH is identical across species
- Assay Duration 15 Minutes
- Samples/Plate 183 in Duplicate (Free GSH), 185 in Duplicate (Total GSH)
- Readout Fluorescent, 510 nm emission / 370-410 nm excitation
- Standard Curve
Assay Principle: The DetectX® Glutathione (GSH) Fluorescent Detection Kit is designed to quantitatively measure glutathione (GSH), and oxidized glutathione (GSSG) present in a variety of samples. The kit is unique in that free and oxidized glutathione are detected in a 384-well microtiter plate. No separation or washing is required. Total glutathione is the sum of GSSG plus GSH. Please read the complete kit insert before performing this assay
Background: Glutathione (L-γ-glutamyl-L-cysteinylglycine; GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 – 10 mM. GSH is a tripeptide molecule. It contains a peptide linkage between the amine group of cysteine and the carboxyl group of the glutamate side-chain. It is an antioxidant, preventing damage to important cellular components caused by reactive oxygen species. Glutathione reduces disulfide bonds formed within cytoplasmic proteins to cysteines by serving as an electron donor. Glutathione is converted to its oxidized form, glutathione disulfide (GSSG). It’s reduced form is most common since glutathione reductase is constitutive and inducible upon oxidative stress. The ratio of reduced glutathione to oxidized glutathione within cells is often used as a measure of cellular toxicity.