• Assay Type Activity Assay
  • Sample Types Serum, Plasma (EDTA and Heparin), Urine, Cell Lysates
  • Sensitivity 2.70 mU/mL
  • Species Human
  • Assay Duration 30 Minutes
  • Samples/Plate 40 in Duplicate
  • Readout Fluorescent, 460 nm emission / 370-410 nm excitation
  • Standard Curve
  • Description

    Assay Principle: 

    The Glutathione S-Transferase (GST) Fluorescent Activity Kit quantitatively measures GST activity in serum, plasma (EDTA and Heparin), urine, and cell lysates. The Glutathione S-Transferase (GST) Fluorescent Activity Kit is an Activity Kit with a run time of 30 minutes. Please read the complete kit insert for more information before performing this assay.

    Use our provided Glutathione S-Transferase Standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a black microtiter plate. Add GST Detection Reagent and Glutathione (GSH) to each well tapping the plate to ensure sufficient mixing of reagents. Then incubate the mixture covered at room temperature for 30 minutes. The fluorescent reaction occurs between the GST Detection Reagent, the GSH, and the GST within the sample or standard. 

    After the 30-minute incubation, use a plate reader to detect and record the generated fluorescent signal at 460nm. Use the intensity and the standard curve to calculate the GST activity in the samples.


    The Glutathione S-Transferase (GST) family of isozymes functions to detoxify and neutralize a wide variety of electrophilic molecules by mediating their conjugation with reduced glutathione. GST enzymes play a pivotal role in alleviating oxidative stress/damage. Several gene families encode Human GSTs, with expression in almost all tissues. GST enzymes are classified as either alpha, mu, pi, sigma, or theta, depending on their structure and substrate specificity. Scientists utilize GST activity as a biomarker for arthritis, asthma, and COPD. The acidic pi class of GSTs is highly prevalent in several human cancers. Furthermore, investigators hypothesize that its activity substantially contributes to the innate or acquired resistance of specific neoplasms to anticancer therapy.