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- Sample Types Serum, Plasma (EDTA and Heparin), Urine, Cell Lysates
- Samples/Kit 40 in Duplicate
- Convenient 30 Minute End Point or Kinetic Assay
- Sensitivity < 100 µU of GST Activity
- Stability Liquid 4ºC stable reagents
- Readout Fluorescent, 460 nm emission / 370-410 nm excitation
- Standard Curve
A GST standard is provided to generate a standard curve for the assay. The kit utilizes a non-fluorescent molecule that is a substrate for the GST enzyme which covalently attaches to glutathione (GSH) to yield a highly fluorescent product. In the end point mode, mixing the sample or standard with the supplied Detection Reagent and GSH yields a fluorescent product which is read at 460nm in a fluorescent plate reader with excitation at 390nm. For rate determination, the intensity at 460nm is read periodically and plotted versus time.
The DetectX® Glutathione S-Transferase (GST) family of isozymes function to detoxify and neutralize a wide variety of electrophilic molecules by mediating their conjugation with reduced glutathione. Human GSTs are encoded by several gene families, and are expressed in almost all tissues. Given its pivotal role in ameliorating oxidative stress/damage, GST activity has been repeatedly investigated as a biomarker for arthritis, asthma, COPD, and multiple forms of cancer, as well as an environmental marker. Examination of GST isoforms and activity in human cancers, tumors and tumor cell lines has revealed the predominance of the acidic pi class. Furthermore, this activity is thought to substantially contribute to the innate or acquired resistance of specific neoplasms to anticancer therapy.