Specifications

  • Assay Type Activity Assay
  • Sample Types Serum, Plasma (EDTA and Heparin), Urine, Cell Lysates
  • Sensitivity 2.70 mU/mL
  • Species Human
  • Assay Duration 30 Minutes
  • Samples/Plate 40 in Duplicate
  • Readout Fluorescent, 460 nm emission / 370-410 nm excitation
  • Standard Curve
  • Description

    Assay Principle: The DetectX® Glutathione S-Transferase Fluorescent Activity Kit is designed to quantitatively measure the activity of GST present in a variety of samples. Please read the complete kit insert before performing this assay

    Background: The DetectX® Glutathione S-Transferase (GST) family of isozymes function to detoxify and neutralize a wide variety of electrophilic molecules by mediating their conjugation with reduced glutathione. GST enzymes play a pivotal role in ameliorating oxidative stress/damage. Human GSTs are encoded by several gene families, and are expressed in almost all tissues. GST enzymes are classified as either alpha, mu, pi, sigma, or theta depending on their structure and substrate specificity.  GST activity has been repeatedly investigated as a biomarker for arthritis, asthma, and COPD. The acidic pi class of GSTs is highly prevalent in several human cancers. Furthermore, its activity is thought to substantially contribute to the innate or acquired resistance of specific neoplasms to anticancer therapy.