Assay Principle:

The DetectX® Cortisone Chemiluminescent ELISA Kit quantitatively measures cortisone in various samples, including serum, plasma, urine, saliva, and fecal extracts. This competitive ELISA features a run time of 2 hours, combining efficiency with accuracy. Read the complete kit insert for detailed instructions before conducting the assay. The kit includes a cortisone standard to establish a precise standard curve.

Protocol Summary:

• Transfer standards or diluted samples into the provided transparent microtiter plate coated with goat anti-rabbit IgG antibody.
• Add cortisone peroxidase conjugate and cortisone polyclonal rabbit antibody to initiate the immunological reaction.
• Incubate the mixture for 2 hours at room temperature with shaking. The reaction varies inversely with the cortisone concentration in the sample.
• After incubation, wash away excess conjugate and add the chemiluminescent substrate, which reacts with the bound conjugate to produce a detectable signal.
• Use a plate reader to measure the signal intensity and calculate cortisone concentration based on the standard curve.

Background:

Cortisone (C21H28O5), also known as Kendall’s Compound ‘E’, was first identified through extraction from bovine suprarenal gland tissue. The concentrations of cortisol and cortisone in the body are regulated by the activity of the two 11β-hydroxysteroid dehydrogenase (11β-HSD) enzymes. 11β-HSD1, predominantly found in the liver, converts cortisone to cortisol, while 11β-HSD2, located in tissues such as the kidney, is essential for cortisol receptor binding.

This glucocorticoid shuttle initiates and regulates the body’s anti-inflammatory response. Understanding the dynamic between cortisol and cortisone impacts research on stress physiology, endocrine disorders, and inflammatory diseases. Additionally, the study of cortisone’s role in metabolic processes and its interaction with various bodily systems continues to be a significant focus in medical research.

The DetectX® Cortisone Chemiluminescent ELISA Kit, with its high sensitivity and specificity, is an important tool for researchers studying these aspects of human physiology and pathophysiology, providing accurate measurements of cortisone levels in a variety of biological samples.