Glutathione (GSH) Fluorescent Detection Kit

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Features

  • USE - Measure Free and Total Glutathione in same well.
  • SAMPLE - Lysate, WB, RBCs, Serum, Plasma, Urine and Tissue
  • SAMPLES/KIT - 39 or 231 in Duplicate
  • STABILITY - Stable 4˚C Liquid Reagents

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Multi Species and Most Sensitive

Description

The DetectX® Glutathione (GSH) Fluorescent Detection kit utilizes a proprietary non-fluorescent molecule, ThioStar®, that covalently binds to the free thiol group on GSH to yield a highly fluorescent product. After mixing the sample or standard with ThioStar® and incubating at room temperature for 15 minutes, the GSH-generated signal is read at 510 nm in a fluorescent plate reader with excitation at 390 nm. Addition of a reaction mixture converts all the GSSG into free GSH, which then reacts with the excess ThioStar® during a second 15 minute incubation, yielding the signal related to Total GSH content.

Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios.

Glutathione Fluorescent Standard Curve

Data Reduction Template by MyAssays

Item Catalog # Price
Glutathione Fluorescent Detection Kit , 1 PlateK006-F1$275.00Inquire About This Product
Glutathione Fluorescent Detection Kit , 5 PlateK006-F5$845.00Inquire About This Product


Publications

Date Publication
Apr 23 2017 Methionine sulfoxide reductase A protects hepatocytes against acetaminophen-induced toxicity via regulation of thioredoxin reductase 1 expression
Apr 20 2017 Preventing, treating, and predicting barbering: A fundamental role for biomarkers of oxidative stress in a mouse model of Trichotillomania
Mar 27 2017 Food supplementation, but not predation risk, alters female antioxidant status during breeding
Mar 08 2017 Oxidative stress in aspic vipers facing pregnancy and water constraints
Mar 02 2017 Oral N-acetylcysteine and exercise tolerance in mild chronic obstructive pulmonary disease
Jan 16 2017 Methionine sulfoxide reductase A deficiency exacerbates acute liver injury induced by acetaminophen
Sep 30 2016 Telomere damage and redox status alterations in free-living passerines exposed to metals
Sep 20 2016 Nitrosopersulfide (SSNO−) targets the Keap-1/Nrf2 redox system
Sep 02 2016 Effect of physical exercise training in patients with Chagas heart disease: study protocol for a randomized controlled trial (PEACH study)
Jun 29 2016 Modulation of MAPK and NF-κB Signaling Pathways by Antioxidant Therapy in Skeletal Muscle of Heart Failure Rats
Mar 28 2016 Murine Lung Cancer Increases CD4+ T Cell Apoptosis and Decreases Gut Proliferative Capacity in Sepsis
Feb 12 2016 Aging is associated with dimerization and inactivation of the brain-enriched tyrosine phosphatase STEP
Jan 19 2016 The Delay of Corneal Wound Healing by Diclofenac in a Human Ex Vivo Front of the Eye Model and Rabbit Models
Oct 20 2015 Increased Energy Demand during Adrenergic Receptor Stimulation Contributes to Ca2+ Wave Generation
Jul 23 2015 Assessment of Protective Role of Multifunctional Dopamine Agonist D-512 Against Oxidative Stress Produced by Depletion of Glutathione in PC12 Cells: Implication in Neuroprotective Therapy for Parkinson’s Disease
Jul 01 2015 Effects of azathioprine, 6-mercaptopurine, and 6-thioguanine on canine primary hepatocytes
May 19 2015 Investigating Endocrine and Physiological Parameters of Captive American kestrels Exposed by Diet to Selected Organophosphate Flame Retardants
Mar 17 2015 Chromosomal damage and EROD induction in tree swallows (Tachycineta bicolor) along the Upper Mississippi River, Minnesota, USA
Jan 20 2015 Valproic Acid-induced Hepatotoxicity in Alpers Syndrome is Associated with mPTP Opening Dependent Apoptotic Sensitivity in an iPSC Model
Jan 04 2015 IS COGNITIVE IMPAIRMENT IN CIRRHOTIC PATIENTS DUE TO INCREASED PEROXYNITRITE AND OXIDATIVE STRESS?
Dec 21 2014 Oxidative stress and mitochondrial dysfunction in Kindler syndrome
Oct 01 2014 P82 - Biomarkers research in neuromuscular disease Charcot-Marie-Tooth
Aug 01 2014 Effect of oral N-acetyl cysteine supplementation in type 2 diabetic patients on intracellular glutathione content and innate immune responses to Burkholderia pseudomallei
Apr 14 2014 Effects of Prolonged Exposure to Hypobaric Hypoxia on Oxidative Stress, Inflammation and Gluco-Insular Regulation: The Not-So-Sweet Price for Good Regulation
Mar 31 2014 Oxidative stress and antioxidant response in fibroblasts from Werner and Atypical Werner Syndromes
Mar 28 2014 Acute nutrient regulation of mitochondrial glutathione redox state in pancreatic β-cells
Mar 20 2014 The Induction of Biochemical Changes in Daphnia magna by CuO and ZnO Nanoparticles
Feb 03 2014 Role of eNOS and Nrf2 in the vascular effects of (-)-epicatechin
Dec 09 2013 Role of antioxidant enzymes in redox regulation of NMDAR function and memory in middle-age rats
Oct 31 2013 Decreased cell proliferation and higher oxidative stress in fibroblasts from Down Syndrome fetuses. Preliminary study
Feb 21 2013 High versus moderate intensity aerobic exercise training effects on skeletal muscle of infarcted rats
Jan 29 2013 Bisphenol A activates Nrf1/2-antioxidant response element pathway in HEK 293 cells
May 29 2012 Mitochondrial oxidative stress contributes differently to rat pancreatic islet cell apoptosis and insulin secretory defects after prolonged culture in a low non-stimulating glucose concentration.
Oct 11 2011 The role of nitrogen oxides in human adaptation to hypoxia
Dec 09 2009 Substitution of serine for proline in the active center of type 2 iodothyronine deiodinase substantially alters its in vitro biochemical properties with dithiothreitol but not its function in intact cells.

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