Protocols
Steroid Tissue Extraction
Protocol for steroid extraction from tissue samples.
Steroid Solid Extraction
Protocol for extraction of steroids from fecal or other non-liquid matrices.
Steroid Liquid Extraction
Protocol for steroid extraction from serum, plasma or saliva or other liquid matrices.
Saliva Sample Handling Protocol
Protocol for processing saliva samples prior to running in DetectX® Assay Kits.
Cyclic Nucleotide Tissue Extraction
Protocol for extracting cyclic nucleotides, cAMP or cGMP (cNMPs), from tissue samples using the Sample Diluent provided in the assay kit.
Peptide/Protein Extraction
Protocol for peptide or protein extraction from matrices such as serum, plasma, tissue, or CSF.
Hair, Feather, and Nail Extraction
Protocol for extraction of steroids, eicosanoids, and peptides from hair, nail, and feather samples.
Eicosanoid Sample Extraction
Protocol for extraction of prostaglandins, thromboxanes, and leukotrienes from biological samples.
Videos
Data Analysis
How to analyze and reduce your data using Arbor Assays’ kits.
Setting up an Arbor Assays ELISA
How to set-up an Enzyme Immunoassay (ELISA) using Arbor Assays’ kits.
Sample Preparation
How to handle various samples prior to running in Arbor Assays’ kits.
Arbor Assays Site Tour
All Arbor Assays products are developed, manufactured, and tested in Ann Arbor, Michigan.
Fecal Extraction Demo
Extraction of fecal samples prior to assay.
Chemiluminescent ELISA Demo
How to set up a chemiluminescent assay with Arbor Assays.
Tail Bleed Sample Prep
Preparation of small volume tail bleed samples for steroid assays.
Assay and Kit Resources
Frequently asked Questions
Publications Search Tool
Search for publications citing Arbor Assays kits by kit, sample type, and species.
K081-F Data Reduction Template
This spreadsheet is designed to be used with Arbor Assays’ DetectX® 2′,3′-cGAMP STING Based FRET Kit, K081-F1/F5.
Saliva-Validated Assays
A listing of Arbor Assays’ saliva-validated ELISA kits for a variety of relevant biomarkers and more information on using saliva as a sample type.
Luminescent Plate Readers
KB02-H1 Data Reduction
An Excel spreadsheet useful for reducing data generated using KB02-H, the DetectX® Serum Creatinine Detection Kit. Used to generate a linear regression standard curve and calculate the concentrations in samples.
K009-F1 Blank Subtraction
An Excel spreadsheet useful for subtracting out thiol background from data generated using K009-F1, the DetectX® Glutathione Reductase (GR) Fluorescent Activity Kit.
Arbor Assays Kit Plate Dimensions
The dimensions for Arbor Assays’ microplates. Useful for when manual adjustment of the reading parameters is necessary for the plate reader to be used.
Links
Organomation
A company that provides sample preparation instrumentation such as those used for evaporation and extraction.
Organic Solvents: Table of Properties
A table of properties for common organic solvents found in the lab.
NIST – Chemistry
A useful resource for chemical properties of common biological molecules.
NCBI Databases – PubMed
A useful database for established concentration ranges for molecules of interest in various species, sample types, and more at NCBI Databases.
MyAssays Data Reduction Templates for DetectX® Assay Kits
Data Reduction Templates for DetectX® Assay Kits.
Mayo Medical Laboratories
Mayo Clinic Reference Laboratory Services site, useful for finding human reference ranges for a variety of analytes in different biological samples. Searchable by test or by disease state.
Linscott’s Directory of Immunological & Biological Reagents
A database connecting researchers to reagent suppliers.
International Society of Wildlife Endocrinology (ISWE)
A non-profit organization aiming to advance the science of wildlife endocrinology. Members develop and apply innovative approaches to study wildlife health, reproduction, welfare, and conservation.
Human Atlas Project
A searchable website providing a Tissue-Based Map of the Human Proteome, achieved through antibody-based methods combined with transcriptomics analysis across all major tissues and organs of the human body.
Luminescent Plate Readers
Lab Math
A walkthrough of lab math calculations involved in sample concentration determinations.
ChemExper
A chemical directory useful for identifying physical and chemical properties, similar or related chemicals, suppliers, etc.
Other
Credit Application
All new customers who wish to place an order by PO are required to submit a credit application form. Please download and complete this form and either e-mail ([email protected]) or fax (+1-734-677-6860) it to our customer service team.