NEW STUDY IN OXIDATIVE STRESS USES ARBOR ASSAYS’ DetectX^® NITRIC OXIDE COLORIMETRIC DETECTION KIT

WHY
Type 2 diabetes mellitus (T2 DM) is one of the most pervasive cardiovascular risk factors contributing to high mortality rate.  Patients with T2 DM develop atherosclerotic plaques in peripheral arteries, driven by endothelial dysfunction and oxidative stress. In … » Read More

In observance of the United States’ Independence Day Holiday, Arbor Assays will be closing early on July 2nd and will remain closed on Monday and Tuesday, July 5th and 6th. Normal business hours will resume on Wednesday, July 7th.

Shipping Guidelines:

Customers:

• Orders placed by Wednesday, June 30th will ship as normal
• Orders placed on July 1st and 2nd will ship on Wednesday, July 7th

Distributors:

• Orders placed by 12:00 p.m.

RAPID & CONVENIENT GLUCOSE MEASUREMENT

WHY STUDY GLUCOSE?

Glucose (C₆H₁₂O₆) is by far the most common carbohydrate. For all biological and molecular events and for multiple cellular functions, energy is essential and is available in the form of ATP (adenosine triphosphate), most of which is generated through aerobic cellular respiration of carbohydrates and glucose. … » Read More

TRAFFIC-RELATED AIR POLLUTION AND A RISE IN CORTISOL LEVELS

WHY?
Prior research has shown that traffic-related air pollution (TRAP) plays a key role in oxidative stress, but its effect on underlying mechanisms, such as glucocorticoid signaling, is still being determined. It is known that glucocorticoids mediate the effects of particulate and gaseous pollutants, but before now, there was no direct evidence of this concerning TRAP.… » Read More

About Cyclic Nucleotides

Cyclic adenosine monophosphate (cyclic AMP or cAMP) and cyclic guanosine monophosphate (cyclic GMP or cGMP) are prolific intracellular signaling molecules. The critical role of cyclic dinucleotides continues to emerge. In mammalian cells, cGAMP is implicated in innate immune response to foreign or harmful DNA.… » Read More

NEW ADRENOCORTICOTROPIC HORMONE (ACTH) ELISA KIT FROM ARBOR ASSAYS

ABOUT ACTH

Adrenocorticotropic hormone (ACTH, adrenocorticotropin, corticotropin) is a polypeptide hormone produced and secreted by the anterior pituitary gland.  It is an important component of the hyphothalamic-pituitary-adrenal axis and in response to stress, it is often produced along with its precursor, Corticotropin-Releasing Hormone from the hypothalamus. … » Read More

Arbor Assays will be closed on Friday, April 2, 2021 for a company holiday.

Any orders placed on Friday will be processed to ship the following business day. A confirmation number with the tracking information will be emailed to you when your order has been scheduled to ship.… » Read More

WHAT’S IN A SEAL’S WHISKER?

WHY?

Scientists are always looking for unique ways to obtain non-invasive tissues for physiological analysis of animals. Most body tissues or fluids such as blood, urine or fecal extracts provide only a glimpse of the reproductive and stress conditions at a given time.… » Read More

FLOWERPOT METHOD DOES NOT INDUCE STRESS

Scientists rely heavily upon the flowerpot method of rapid eye movement sleep (REMS) deprivation (REMSD) to study the functions of REMS. This system keeps experimental animals on raised platforms over water, where they are unable to relax and enter REMS. The… » Read More

INTRODUCING: A NEW PARTNERSHIP WITH ONE TREE PLANTED

In 2021, Arbor Assays will be donating one tree to the organization One Tree Planted for every kit we sell, and we hope to reach our goal of 10,000 trees planted by January 2022!… » Read More

SENSITIVE ASSAYS FOR CYCLIC NUCLEOTIDE SIGNALING

Cyclic adenosine monophosphate (cyclic AMP or cAMP) and cyclic guanosine monophosphate (cyclic GMP or cGMP) are prolific intracellular signaling molecules. The critical role of cyclic dinucleotides continues to emerge. In mammalian cells, cGAMP is implicated in innate immune response to foreign or harmful DNA.… » Read More

COVID-19 AND ACUTE KIDNEY INJURY

COVID-19, the disease caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), often causes lasting lung damage. However, coronavirus can also cause severe and lasting harm in other organs, including the heart and kidneys.  People suffering with severe cases of COVID-19 are showing signs of kidney damage, and reports say up to 40% of patients hospitalized with COVID-19 develop moderate or severe kidney injury (1-3).… » Read More

ANNOUNCING OUR 2021 RESEARCH THE CURE™ PICK: BRAIN CHEMISTRY LABS

Arbor Assays is proud to announce that for our 2021 Research the Cure™ program, we will be donating proceeds from every kit purchased to Brain Chemistry Labs!

“…We care only for patients, not profits.”

IS GINGER AN ANCIENT WONDER DRUG?

Ginger (Zingiber officinale) is one of the most commonly consumed spices in the world and has been used for thousands of years for the treatment of numerous ailments, such as colds, nausea, arthritis, migraines, and hypertension.… » Read More

KEEPING YOUR ELEPHANTS HAPPY

A new longitudinal study from ISWE members.

A basic understanding of wildlife endocrinology is critical to ensure the health and welfare of endangered species. Hormone analysis is a widely accepted tool for monitoring reproductive function and responses to stressors in captive and free-ranging animals. Understanding… » Read More

This holiday season, Arbor Assays will be closed Thursday, December 24th, 2020 through Friday, January 1st, 2021. We will resume normal business hours on Monday, January 4th, 2021.

The holiday shipping schedule is outlined below:

• The final shipping day for U.S.

HOW DOES DIET REDUCE STRESS?

It’s not a secret that diet can be part of an effective strategy for controlling stress. A healthy diet helps counter the impact of stress by boosting the immune system and lowering blood pressure. Though the importance of diet for stress reduction is well known, the mechanisms at play still require further investigation.… » Read More

NATURAL PRODUCTS AND ANTI-INFLAMMATORY PROPERTIES

Natural products have long been used in traditional medicine for their unique anti-inflammatory and antioxidant properties and many complex natural products have become successful pharmaceutical agents.  However, the potential of many natural products has not been fully realized because they tend to exhibit physiochemical properties that result in low membrane permeability.… » Read More

CAN CANNABIS REALLY REDUCE STRESS?

Cannabis has long been used for stress reduction, and its anxiolytic and anti-depressive abilities are generally well accepted. However, acute THC administration has also been shown to increase concentrations of stress glucocorticoids like cortisol and to diminish cortisol reactivity to stress^1,2.… » Read More

SENSITIVE ASSAYS FOR CYCLIC NUCLEOTIDE SIGNALING

Cyclic adenosine monophosphate (cyclic AMP or cAMP) and cyclic guanosine monophosphate (cyclic GMP or cGMP) are prolific intracellular signaling molecules. The critical role of cyclic dinucleotides continues to emerge. In mammalian cells, cGAMP is implicated in innate immune response to foreign or harmful DNA.… » Read More

Lizards Are Feeling the Heat:

Body temperature and stress adaptation in ectotherms

Survival in the wild requires quick adaptation to stressful events. Activation of the hypothalamic-pituitary-adrenal (HPA) axis and short-term elevation of glucocorticoid hormones help animals deal with stress and maintain homeostasis.… » Read More

New treatments for neurodegenerative diseases: On the horizon?

Chronic neuroinflammation plays a critical role in the onset and progression of a broad range of neurodegenerative diseases such as Huntington’s disease, Parkinson’s disease, and multiple sclerosis.  CNS inflammation is mostly driven by activation of resident microglia, which normally acts as the first line of defense against injury and invading pathogens. … » Read More

Chronic repeated unpredictable stress induces PTSD in mice… and likely humans, too.

Chronic stress can lead to dysregulation of hypothalamic-pituitary-adrenal (HPA) axis function, and is implicated in diseases like depression and post-traumatic stress disorder (PTSD). The development of treatments for stress-related disorders requires a better understanding of the biological basis of HPA axis dysfunction. … » Read More

Our Science in September

The time you spend in the lab is valuable.

That’s why we’ve compiled some resources and services that we hope will help you in your lab and throughout your research.

Custom Sample Testing Services

Let our experts analyze samples for you and get your research back on track. … » Read More

Social Interaction and Outdoor Activity Boost Testosterone Levels in Captive Bull Elephants

Wild African elephant populations are rapidly declining due to habitat loss and illegal poaching. Given the uncertain future for elephants in the wild, zoos play an important role in their conservation, and successful breeding programs are critical for maintaining the genetic variability in captive elephant populations.… » Read More

National Zoo Gains a Baby Panda

PGFM Assay a Hero in Predicting Time of Birth

The overjoyed zoo staff reported their resident Giant Panda, Mei Xiang, gave birth on August 21st, 2020, and you can watch both Mei Xiang and her new cub in real time on their Panda Cam!… » Read More

Non-invasive fecal hormone analysis for stress and reproduction assessment in wildlife studies

Hormone levels are widely used to measure stress and reproductive status in humans and animals. Levels of progestins and estrogens are used to measure ovulation and reproductive cycle, while glucocorticoids are reliable biomarkers of both physiological and psychological stress.… » Read More

Study finds Sodium Thiosulfate reduces Doxorubicin (DOX)-induced DNA damage

Doxorubicin (DOX), commonly used to treat a variety of cancers, has a number of adverse side effects including acute cardiotoxicity. DOX-induced cardiac dysfunction is due to reactive oxygen species (ROS)-mediated oxidative stress and subsequent apoptosis of cardiomyocytes. … » Read More

The DetectX^® Arg⁸-Vasopressin Colorimetric ELISA Kit (K049-H1/H5) provides a simple, fast, and user-friendly protocol to measure the AVP in biological samples.

KIT DETAILS

• Samples: Measure AVP in serum, plasma, and tissue culture media
• Sensitivity: 3.7 pg/mL
• Stable: 4°C stable, liquid, ready-to-use reagents
• Convenient: Extraction reagent included, no C-18 SPE columns needed
• Multi-Species: Measure AVP in mammals and Arg-Vasotocin in birds and reptiles
• Cost-Effective: 1- or 5-plate kit – up to 39 or 231 samples in duplicate

The ELISA protocol is an easy, cost-effective method and widely used for routine analysis of AVP in clinical, biological, and behavioral research laboratories.… » Read More

Reactive Oxygen Species (ROS) can react with vital macromolecules, damaging biomolecules critical to cellular structure and function. Antioxidants protect biological systems from ROS-induced oxidative damage, and populations with diets high in antioxidant phytochemicals (polyphenols, flavonoids, etc.) tend to have lower incidence rates of chronic diseases associated with oxidative stress.… » Read More

The negative impact stress has on health and performance is well documented. Consequently, stress assessment and mitigation strategies are the subject of ongoing study. Cortisol is often considered the gold-standard biomarker in clinical sciences and psychology, and cortisol levels are commonly measured to assess stress levels in humans and animals.… » Read More

The time you spend in the lab is valuable.

Due to the COVID-19 pandemic, much of this time was cut short. Now, as you slowly make your way back into the lab, there are a few questions that need to be answered: How do you prepare for this return?… » Read More

Regulation of Copper Transport and Cell Viability During Copper Metabolism

Copper (Cu) is an essential cofactor in virtually all organisms, and tight control of Cu metabolic pathways are critical for optimal cellular viability.  Cu is required for proper function of a variety of important enzymes, such as cytochrome c oxidase, ceruloplasmin, and Cu/Zn superoxide dismutase.… » Read More

Glutathione, the major endogenous antioxidant produced by cells, plays a critical role in protecting organisms from oxidative stress and related cytotoxicity. The ratio of reduced (GSH) and oxidized (GSSG) glutathione is an important measure of a cell’s ability to maintain redox homeostasis.… » Read More

Arbor Assays’ Newest Oxidative Stress Assay

Lipid peroxidation is one of the most commonly reported indices of oxidative stress and has been implicated as a contributing factor in a range of degenerative diseases, including diabetes¹, cardiovascular disease, Parkinson’s disease, Alzheimer’s disease², and psychiatric disorders, such as schizophrenia³. … » Read More

Stress at the Cellular Level

Cells maintain an equilibrium of molecular oxygen and various antioxidants. An imbalance of reactive oxygen species (ROS), which form as reaction intermediates in normal aerobic life, can lead to cell toxicity. Excess oxygen radicals can overwhelm the antioxidant capacity leading to oxidation of macromolecules such as lipids, proteins, and DNA.… » Read More

Arbor Assays’ Corticosterone ELISA Kit is known as the only kit on the market that measures corticosterone in as little as 2 μL of serum or plasma in just 90 minutes.

Now, it’s even better:

• Improved sensitivity with Multi-Format option:
  • 50 μL format from 10,000 to 39.06 pg/mL
  • 100 μL format from 5,000 to 19.53 pg/mL
• Saliva samples now validated.

We know the cGAS–STING pathway is a key activator of the innate immune response to cellular damage or microbial infection. Cyclic GMP-AMP Synthase (cGAS) catalyzes the formation of cyclic GMP-AMP (cGAMP) in  response to the presence of foreign DNA in the cytoplasm.… » Read More

Dear Customers and Partners,

I wanted to make you aware of the steps Arbor Assays is taking due to the Michigan Stay Home, Stay Safe Executive Order announced this morning by Governor Whitmer in response to the spread of COVID-19 coronavirus.… » Read More

Production of reactive oxygen species (ROS) like superoxide (O^–₂·), hydrogen peroxide (H₂O₂), and hydroxyl radicals (•OH) is a normal part of life. Moderate concentrations are involved in a number of signaling pathways, synthesis of cellular structures, and host defense against pathogens. … » Read More

STUMPED?
The molecules, of course, are 100% identical. The structure of the cortisol molecule is exactly the same regardless of the species or sample where it’s found.

Q: SO, WHY ARE THERE SO MANY SPECIES-SPECIFIC CORTISOL ASSAYS ON THE MARKET?

A: BECAUSE THEY DON’T KNOW STEROIDS LIKE WE DO.… » Read More

Early-life stressful experiences can have negative consequences on brain maturation. To understand the long-term implications of neonatal stress, researchers from Wayne State University’s Department of Psychology investigated the biobehavioral consequences of neonatal pain and maternal isolation in a rat model of neonatal stress.… » Read More

Cyclic dinucleotides are the most abundant second messengers in bacteria, modulating a variety of cellular activities in prokaryotic cells.  3’3’-cyclic GAMP (cyclic [G(3’,5’)pA(3’,5’)p], previously known as cGAMP) is a key mediator of bacterial signal transduction and regulation, controlling a range of diverse targets including transcription, enzyme activity and cell cycle progression. … » Read More

The presence of DNA in the cytoplasm of eukaryotic cells is an alarm signal indicating cellular damage or microbial infection. The cGAS-cGAMP-STING DNA sensing pathway is a key activator of the innate immune response to foreign or harmful native DNA. In the current model, cyclic GMP-AMP Synthase (cGAS) catalyzes the formation of cyclic GMP-AMP (cGAMP) in response to double-stranded DNA binding in the cytoplasm.  … » Read More

Arbor Assays is proud to announce that for our 2020 Research the Cure™ program, we will be donating proceeds from every kit purchased to the Packard Center.

About the Packard Center

Founded in early 2000 at John’s Hopkins, the Robert Packard Center for ALS Research has been committed to researching a cure for this debilitating disease.… » Read More

Stroke is the fifth leading cause of death in the United States and is a major cause of serious disability for adults. Post-stroke dementia (PSD) is the major form of dementia in the elderly. Of the 800,000 people in the US that have strokes every year, about 10% of first-time stroke victims will suffer from PSD, while 30% of recurrent stroke patients suffer from this type of dementia.… » Read More

“Glucocorticoid hormones are secreted with a prominent daily rhythm, but their diurnal genomic actions remain uncharacterized. Quagliarini et al. show how the Glucocorticoid Receptor controls daily cycles of glucose and triglyceride metabolism by time-dependent chromatin binding and target gene transcription, and how this rhythm is augmented by high fat diet.… » Read More

Arbor Assays is pleased to announce our sponsorship of the upcoming annual meeting of the Society of Integrative & Comparative Biology (SICB). We are excited to support the SICB mission to advance research, education and public awareness of organismal, functional, and evolutionary biology.… » Read More

The improved DetectX® Atrial Natriuretic Peptide (ANP) ELISA Kit (K071-H1/H5) from Arbor Assays provides a more sensitive and reliable method for precise quantitation of ANP in a wide range of sample types. The 96-well assay kit measures ANP in urine, plasma extracts, or tissue culture media using a competitive ELISA format in a 90 minute assay.… » Read More

Today, almost 17,000 species are threatened with extinction. This number encompasses only those species that are assessed and well studied. Endangered Species International estimates there are 10 million species awaiting discovery as humans destroy natural ecosystems and habitats. The number of species that are severely threatened will likely surpass today’s 17,000 estimate.… » Read More

C-Reactive Protein (CRP) is a ring-shaped, pentameric serum protein produced by the liver. As an acute phase inflammation marker released in response to IL-6 signaling by T-cells, macrophages, and adipocytes, CRP levels rise in a wide range of acute and chronic inflammatory conditions.… » Read More

Ann Arbor, MI (November 14, 2019) — Arbor Assays adds two new potent STING inhibitors, H-151 and DMXAA, to their catalog of inflammation kits and reagents. The addition of the STING inhibitors combined with Arbor Assays 2’,3’-Cyclic GAMP ELISA Kit provides important new tools for biomedical research and drug discovery.… » Read More

Statistics from the National Institute of Mental Health show almost 20% of all adults in the United States suffer from an anxiety disorder, with a higher prevalence in women (23.4%) than men (14.3%). Anxiety disorders are more prevalent in adolescents, with 38% of females and 26% of males suffering from some sort of disabling anxiety.… » Read More

Wildlife studies and conservation efforts have been increasing in importance as the imperatives of species and environmental management have been recognized worldwide. Those efforts require partnerships with the research community, adaptable research tools, and support from organizations that are focused on preserving the natural world for future generations – organizations like Arbor Assays.… » Read More

Our Allopregnanolone ELISA Kits, K044-H & K044-C, have been discontinued as of October 1, 2019. These kits have been replaced with our Allopregnanolone ELISA Kit, Monoclonal Antibody Based, K061-H. The reason for the discontinuation is an improved version that utilizes a monoclonal detection antibody replacing the polyclonal previously used in the discontinued versions of the kit.… » Read More

Allopregnanolone (5α-pregnan-3α-ol-20-one, Allo)

• Allopregnanolone is released by the adrenal gland in response to stress providing delayed negative feedback inhibition of the HPA axis and promoting anxiolytic and anesthetic effects.
• Allopregnanolone activates GABA receptors with effects similar to those of other positive allosteric modulators of the GABA action such as benzodiazepines, including anxiolytic, sedative, and anticonvulsant activity.

Nitric oxide (NO) is a diffusible, transient, reactive molecule, and an important regulator of cardiovascular, neurological, and immunological systems. NO is generated endogenously by constitutive or induced enzymes like Nitric Oxide Synthase or orally ingested as nitrates/nitrites for rapid uptake into circulation and subsequent conversion.… » Read More

Reliable pregnancy diagnosis in captive breeding programs can be challenging. Accurate, non-invasive biomarkers are vital for successful reproductive management of livestock and endangered wildlife. PGFM has been shown to be a practical reproductive status biomarker for several species.

Accurate reproductive monitoring is essential in wildlife conservation efforts and livestock management operations.… » Read More

Prebiotics are indigestible food fibers and natural sugars that stimulate the growth of healthy bacteria in the gut. Xylooligosaccharides (XOS) are sugar fibers naturally found in the xylan fraction in plant fiber and they are made up of D-xylose oligomers. Due to its highly bonded structure and composition, XOS are stable at low pH and resistant to high temperature.… » Read More

For the twelfth consecutive year as Arbor Assays along with family and friends, participated in Walk MS on May 4, 2019 to raise awareness and funds to support the National Multiple Sclerosis Society. The rain held off for the walk at Monument Park in Dexter, MI!… » Read More

A spinal cord injury (SCI) may occur due to a sudden blow to the spine causing vertebral fractures, compressions or dislocations. An SCI can also damage the spinal cord itself, which is a collective bundle of nervous tissues, causing muscle and body coordination issues and sometimes paralysis.… » Read More

Visiting a whale habitat and seeing these magnificent creatures up close is a wonderful experience. Free-swimming whales, including Humpbacks, are an eye-catching species for sightseeing. In past years, a huge decline in the number of humpback whales has been observed, mostly due to human impact.… » Read More

The C57BL/6 mouse strain, also known as “black 6”, was developed at the Bussey Institute from C.C. Little’s C57BL strain originally created in the 1920s. These dark-coated mice are a popular model organism for a variety of biomedical research studies. The dark coat makes this a convenient strain for creating transgenic mice as they can be crossbred with congenic light coated 129 mice to produce disease models.… » Read More

A recent study by Gao and Matsumoto in the Journal of Ethnopharmacology (S0378-8741(18)34424-6) reported significant reduction in Autism Spectrum Disorder (ASD)-like behaviors in mice treated with Kami-shoyo-san (KSS), a traditional Japanese herbal medicine.

ASD includes a range of neurodevelopmental disorders where affected individuals are often hyper-sensitive to sensory stimuli, have problems with social interaction, and exhibit restricted, repetitive patterns of behavior.… » Read More

2′,3′-Cyclic GAMP Direct EIA Kit
The innate immune system is the body’s crucial first defense against viral infections and it is dependent upon a group of pattern recognition receptors. cGMP-AMP Synthase (cGAS) responds to the presence of viral DNA in the cytosol by producing 2’,3’ cGAMP.… » Read More

A recent study from the Western University of Health Sciences found strong evidence for the anti-mycobacterial potential of flavonoids on tuberculosis infection in at-risk populations.

Tuberculosis (TB) is the leading cause of death by infectious diseases with an estimated one-quarter of the world’s population testing positive for Mycobacterium tuberculosis (Mtb).… » Read More

While oxidative damage to DNA is an inevitable byproduct of cellular metabolism, excess DNA oxidation is frequently associated with carcinogenesis, neurological disorders, and cardiovascular disease.

Free radicals and reactive oxygen species (ROS) are produced during normal cellular metabolism and also by ultraviolet or ionizing radiation.… » Read More

Marine wildlife researchers prefer minimally invasive methods when collecting samples for endocrine studies. Samples like respiratory vapor (‘blow’) and blubber are commonly used but have yet to be validated for these purposes for many cetacean species. A recent study reported by Mingramm and Keeley in General and Comparative Endocrinology examined the performance of blow and blubber hormone monitoring for evaluating the reproductive and adrenal condition of captive bottlenose dolphins.… » Read More

Arbor Assays is pleased to announce the immediate release of the newest assay kit for reproductive biology research. The DetectX^® Progesterone Metabolite Immunoassay Kit, K068-H1/H5, was developed to quantitatively measure Progesterone metabolites present in extracted fecal samples and urine.… » Read More

Along with its well-known function combating oxidative stress through its role as a critical source of reducing power, Glutathione (GSH) is involved in a number of diverse functions including apoptosis, disulfide bond formation, detoxification, antioxidant defense, maintenance of thiol status, and modulation of cell proliferation.… » Read More

Japanese researchers observe anti-stress and antioxidant effects of raw cane sugar.

Modern life can be somewhat stressful and excessive stress is related to a wide variety of diseases. It is thought certain foods can help relieve stress through antioxidant activity and by suppressing stress biomarkers in serum.… » Read More

The newly improved DetectX^® Protein Kinase A Colorimetric Activity Kit (K027-H1) from Arbor Assays provides a sensitive and reliable method for screening inhibitors or activators of PKA and for quantitating PKA activity in a wide range of sample types. The 96-well format kit utilizes a synthetic peptide as a substrate for PKA and a rabbit monoclonal antibody that recognizes the phosphorylated form of the substrate with a three hour assay.… » Read More

Kimchi, a staple in Korean cuisine, is a famous traditional side dish made from salted and fermented vegetables, most commonly napa cabbage and Korean radishes. Kimchi has been regarded worldwide as a functional health food, and the antioxidant, antiobesity, and antidiabetic activities of kimchi have been widely studied.… » Read More

Arbor Assays is proud to offer our Research the Cure™ program for the 8th consecutive year. The program helps support important medical research programs. This year proceeds from each kit purchased will support the Scleroderma Research Foundation as part of our Research the Cure™ program.… » Read More

Compounds found in brightly colored vegetables offer protection from oxidative stress related diseases through increasing Glutathione Peroxidase Activity.

A recent University of Barcelona study by Vera et. al. used Arbor Assay’s Glutathione Fluorescent Detection Kit, K006-F to demonstrate significant inhibitory effect of endothelial cell activation and reduction of reactive oxygen species with an in vitro model of uremia.… » Read More

A recent study by Wojtusik et. al., at the Cincinnati Zoo measured hippopotamus salivary progesterone levels to determine the pregnancy status and likely due dates.

Careful reproductive management is essential to maintain sustainable populations of hippos outside their native ecosystems.… » Read More

Italian researchers demonstrate the health benefits of chocolate consumption.

A recent study by Cavaretta et al. from the Mediterranean Neurological Institute and the University of Rome shows the effect of dark chocolate consumption on biomarkers of oxidative stress and muscle damage in a randomized controlled trial. … » Read More

It is well documented that alcohol during pregnancy can cause complications for pregnancy and birth, but little is known about the effects of parental binge ethanol (EtOH) drinking on future pregnancy and offspring.

A previous study by Asimes et al, 2017 showed that parental binge EtOH drinking caused modifications to the DNA of their progeny; however, the phenotypical and hormonal alterations in the offspring born to binge EtOH-exposed parents were not explored at that time.… » Read More

Post-mitotic neuronal cells are more sensitive to the concentration of reactive oxygen species (ROS) in their surroundings than other cells. To remove excess amounts of deleterious ROS, neurons rely on oxidative phosphorylation and antioxidants such as glutathione (GSH). Previous studies have observed reduced levels of GSH in neuronal cells during neurological disorders, cognitive impairments and aging.… » Read More

Arbor Assays is proud to support the Warrior-Scholar Project at Yale University for a second year. We provided the Warrior-Scholar Project with Cortisol EIA Kits (K003-H) for use in the second week of the program during the Hormones and Behaviors course. … » Read More

NOW AVAILABLE

World’s First 2′,3′ Cyclic GAMP Direct EIA Kit from
Experts in Cyclic Nucleotide Assays

2′,3′ Cyclic GAMP Direct EIA Kits, Catalog No:  K067-H1/H5

FEATURES

• Use: Measure cGAMP in STING signaling
• Range: 50 pmol/mL – 0.205 pmol/mL
• Time to Answer: 2.5 hours
• Samples/Kit: 39 or 231 in duplicate
• Stability: Liquid 4°C Stable Reagents

The innate immune system is the body’s crucial first defense against viral infections and it is dependent upon a group of pattern recognition receptors.… » Read More

Even with significant protections in place, whale populations continue to be low relative to apparent historic norms.   Unfortunately, the reasons for low population and slow population growth have proven to be elusive.  Wild whales are difficult subjects for study.  Samples are hard to obtain, collection stressful for the whales, and getting repeated samples from the same individual over time is extremely rare.  … » Read More

Monogamous primates living in small family groups often exhibit delayed patterns of reproduction.   In many species, females will not conceive until after they have left the family group into which they were born and become established within a new group.   There are a number of theories as to why this may happen, and through what means reproduction is suppressed. … » Read More

A great deal of attention is paid to glucocorticoid levels in connection with the conservation of animal species in the wild or in captivity.  However, monitoring the stress levels of laboratory test animals such as mice and rats can also be critical to understanding the results of the experiments in which they are used. … » Read More

New Kit Coming in May of 2018!

FEATURES:

Use: Measure 20-hydroxyecdysone from a variety of species
Broad Range: 25,000 pg/mL – 100 pg/mL
Time to Answer: 2.5 hours
Specificity: Highly Specific to 20-hydroxyecdysone, very low cross reactivity with other steroids
Ecdysones are a group of steroid hormones that regulate metamorphosis, cell death, and reproduction in arthropods.… » Read More

Arbor Assays is Pleased to Announce the World’s First Cyclic GAMP EIA Kit for
STING Signaling Research.
Coming in May 2018.

Cyclic GAMP EIA Kit, Catalog No:  K067-H

FEATURES

• Use: Measure cGAMP in STING signaling
• Range: 50 pmol/mL – 0.205 pmol/mL
• Time to Answer: 2.5 hours
• Samples/Kit: 39 or 231 in duplicate
• Stability: Liquid, 4°C Stable Reagents

The innate immune system is the body’s crucial first defense against viral infections and it is dependent upon a group of pattern recognition receptors.  … » Read More

Sleep disturbances, including sleep fragmentation, are known to occur more frequently in individuals with type 2 diabetes than in the general population.   Poor sleep has been shown to negatively impact glycemic control and may exacerbate negative metabolic outcomes when combined with other risk factors.… » Read More

The normalization of specific quantitative assay results is critical to interpretation of biological system status. With cells grown in culture and lysed, a simple total protein determination such as the Bradford assay developed by Marion Bradford at the University of Georgia in 1976 will give an estimate of cellular protein.… » Read More

Interaction between the serine/threonine protein phosphatase calcineurin and the NFAT family of transcription factors plays a critical regulatory role in the regulation of a variety of physiological functions.   Calcineurin inhibitors are widely used as drugs to suppress inflammation and immune response, including protecting organ transplant recipients from rejection. … » Read More

“If we are to have peace on earth, our loyalties must become ecumenical rather than sectional. Our loyalties must transcend our race, our tribe, our class, and our nation; and this means we must develop a world perspective.”

Martin Luther King Jr.,

Tuberculosis (TB) remains one of the leading causes of morbidity and mortality worldwide, while response to the only available vaccine (BCG) varies widely with a number of factors.  In the majority of cases, TB infection in otherwise healthy individuals is rendered latent by the formation for granulomas. … » Read More

• MULTI-SPECIES: Mammalian, bird, fish
• SENSITIVITY: >3 times the sensitivity of any other kit
• SIMPLE: No C18 column purifications
• INEXPENSIVE: Extraction solution included
• SAMPLES/KIT: 38 or 230 in duplicate
• STABLE: 4°C Stability

The neuropeptides oxytocin and vasopressin were isolated and synthesized by Vincent du Vigneaud at Cornell Medical College in 1953, work for which he received the Nobel Prize in Chemistry in 1955.… » Read More

Under normal cellular conditions there is a constant balancing act between the creation of reactive oxygen species (ROS) and their elimination by the antioxidant system including enzymes such as superoxide dismutase, glutathione peroxidase, and catalase.  Abnormally low levels of ROS can impede cellular signaling and some normal intracellular reactions, while overly high levels of ROS create oxidative stress which can lead to the improper oxidation of lipids, proteins and DNA resulting eventually resulting in apoptosis, necrosis, and other cellular damage.… » Read More

Assay Features:

• Use:                          Non-Invasive Androgen Determination
• Sample:                    Dried Fecal Extracts Urine, Extracted Serum/Plasma, TCM
• Species:                   Species Independent
• Time to Answer:       2.5 Hours
• Samples/Kit:             39 or 231 in Duplicate

Testosterone, C₁₉H₂₈O₂, (4-Androsten-17ß-ol-3-one) is a steroid hormone from the androgen group and is found in mammals, reptiles, birds, and other vertebrates.… » Read More

Acute inflammation is commonly associated with activation of the HPA axis leading to higher levels of circulating glucocorticoids.  However, studies have indicated that some persistent inflammatory conditions, including rheumatoid arthritis, are associated with abnormal HPA responses. What causes this abnormal HPA axis response is not currently understood.  … » Read More

Empathy is perhaps the most human of all human traits, but relatively little is understood about how empathy works on a biochemical and neurological level.   In a recent paper by Pisansky et al. the authors examine the phenomenon of fear-contagion, an empathy-like response in mice, and whether oxytocin might play a role in how animals react to the stress of others.… » Read More

Mining is an inherently invasive and destructive activity with broad ranging and long term effects on the environment.  Despite advances in regulation and oversight, pollution remains a significant and long term problem.

A recent paper by Beyer et al. examined the effects of environmental heavy metal contamination from mine tailing and run off on the white-footed mouse (Peromyscus leucopus).  … » Read More

Dehydroepiandrosterone (DHEA) is the most abundant circulating C19 steroid in humans. Although DHEA itself has very low androgenic activity, it is the major intermediate in the biosynthesis of the more potent androgen and estrogen sex steroids. DHEA is secreted primarily by the adrenal glands, but may also be produced in the gonads and brain.… » Read More

Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role.  Normally stored in azurophilic granules, MPO is released into the extracellular space during degranulation. There, as part of the neutrophils “respiratory burst” it produces hypochlorous acid from hydrogen peroxide and Cl^–.… » Read More

As human population grows, our species continues to expand into habitats previously occupied by other species around the globe.  Unfortunately, we do not make very good neighbors.  The impact of human industry and occupation are diverse and devastating.  In addition to the obvious physical habitat lost to concrete and paving, there is further harm done by chemical, noise and light pollution, introduction of non-native species, and increased spread of diseases.  … » Read More

Ceruloplasmin (Cp) is a blue-colored glycoprotein that contains six copper ions per molecule. It is an acute phase protein – concentrations increase in response to inflammation – and also functions as the main copper transport system in the body. Copper is incorporated into the molecule when it is synthesized by heptaocytes, and released when the Cp is catabolized.… » Read More

The cytochrome P450 (CYP) superfamily of heme-containing enzymes displays great diversity with regard to substrate specificity and catalytic activity. P450s use a plethora of both exogenous and endogenous compounds as substrates in enzymatic reactions, often functioning as the terminal oxidase enzymes in electron transfer reactions (see figure below).… » Read More

Aldosterone is a steroid hormone primarily involved in the regulation of sodium and potassium levels in blood as part of the renin-angiotensin system (RAS). Aldosterone increases reabsorbation of sodium from the tubular epithelial cells of kidneys back into the blood, while at the same time moving potassium ions from the blood into the kidneys for eventual excretion in urine.… » Read More

Much of what is currently known about the reproductive physiology of wild equine species is based on behavioral observation or data collected from culled individuals.  It is tempting to fill in gaps in knowledge of a rare species by assuming similarity to a more common relative we know more about, however this can be misleading as even closely related species may not exhibit similar reproductive patterns.  … » Read More

Arbor Assays is the World’s Most Trusted Source for Corticosterone Assay Kits!

View over 200 Citations for our Corticosterone EIA Kits

Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland in response to stimulation by adrenocorticotropic hormone.… » Read More

Induced pluripotent stem cells are a powerful tool for investigating the molecular mechanisms that underlie cell development in vitro.  A November 2017 paper by Matsuo et al. examined the process of steroidogenic differentiation using human stem cell derived intermediate mesoderm cells known to be the origin of human adrenal cortex and gonads. … » Read More

The cholinesterase family of enzymes hold important roles in a variety of bodily functions. Acetylcholine esterase (Acetylcholinesterase, AChE) is critical for CNS functions, whereas butyrylcholine esterase (Butyrylcholinesterase, BChE) is a nonspecific cholinesterase enzyme that hydrolyzes many different choline-based esters.

The use of AChE inhibitors as therapeutic agents and pesticides has spurred detailed investigations of cholinesterases after the initial physical characterization of the enzyme by Leuzinger in the late 1960’s (2).… » Read More

The majority of conservation efforts across the globe are based on the well-studied distribution of mammal, bird, and amphibian species, forming much of what we currently understand about global biodiversity.   However, a recent paper in Nature Ecology and Evolution by U.… » Read More

Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules. This process is held in check by the existence of multiple antioxidant and repair systems, as well as the replacement of damaged nucleic acids, proteins and lipids.… » Read More

Methylation and demethylation of cellular proteins and DNA/RNA plays a significant role in the regulation of a variety of signal transduction pathways including protein translation and complex energy transfer systems

Because the enzymes responsible for methylation and demethylation reactions are deeply intertwined with the systems they regulate, these enzymes can be especially tricky to study and understand.  … » Read More

Monogamous primates living in small family groups often exhibit delayed patterns of reproduction.   In many species, females will not conceive until after they have left the family group into which they were born and become established within a new group.   There are a number of theories as to why this may happen, and through what means reproduction is suppressed. … » Read More

Because we believe in giving back to our community, Arbor Assays makes a charitable donation each year based upon the number of kit products sold within that year.  In a program we call “Research the Cure™”, this donation goes directly to a charity supporting medical research chosen by the Arbor Assays employees.  … » Read More

Even with significant protections in place, whale populations continue to be low relative to apparent historic norms.   Unfortunately, the reasons for low population and slow population growth have proven to be elusive.  Wild whales are difficult subjects for study.  Samples are hard to obtain, collection stressful for the whales, and getting repeated samples from the same individual over time is extremely rare.  … » Read More

Hemoglobin is an iron containing, multi-subunit protein responsible for transporting oxygen from the respiratory organs to the rest of the body tissues in vertebrates (with the exception of fish from the family Channichthyidae – which use a different oxygen transport system).… » Read More

Use of nanoparticles in a wide range of consumer and industrial applications is already widespread and expected to increase exponentially during the next decade.   While the properties of the corresponding bulk materials may be familiar and well understood, nanoparticles have unique physiochemical properties and little is currently understood about their long term impact on health and the environment. … » Read More

Human beings have been altering our environment since prehistoric times and these changes impact human health and development in many different ways. In modern times, the levels of chemical environmental contaminates that the average human is exposed to have been increasing at an incredible rate with significant impact on health.… » Read More

The ability of cells to perceive and correctly adapt to their microenvironment is critical for maintaining normal homeostasis and development as well as for appropriate response to external stimuli. Receptors on the cell surface bind extracellular signaling molecules and transfer that signal to the interior of the cell, setting in motion a cascade of enzymatic reactions and secondary messenger molecules, ultimately altering cell function in response to the extracellular message.… » Read More

As seeds age, their ability to germinate declines even if stored under optimal conditions. This loss of production potential is an obstacle to agricultural production and to germplasm and species conservation.  Understanding how this occurs and potentially preventing or slowing the loss of seed vigour is of great interest to plant researchers.… » Read More

Domestic dogs are highly social pack animals with an advanced ability to form complex relationships and to read and interpret human behavior, innately so from the first trial with little to no learning curve regardless of age or rearing.  They display a strong attachment for their owners and other familiar people that studies have shown to be analogous to human attachment behaviors.… » Read More

Features

• Two format options in one kit
• Fast: Results in 90 minutes
• Stable: Liquid reagents stable at 4°C
• Economical: 38 or 230 samples in duplicate
• Validated sample types: Serum, Plasma, Urine and Dried Blood Spots

The World Health Organization has estimated 250 million children have moderate to severe Vitamin A deficiency (VAD) due to lack of adequate nutrition and the rising cost of food staples around the world further exacerbates this problem.… » Read More

• As Little as 1 μL Serum or Plasma Sample Required for Duplicate Measurements
• Proven Results  – Over 200 Literature Citations for EIA and CLIA
• No extractions necessary
• Extended Range – 10,000 to 78 pg/ml (EIA) or 3000 to 12.3 pg/ml (CLIA)
• Results in 90 minutes (EIA) or 2 hours (CLIA)
• Multispecies – Validated in mice, rats, monkeys, other mammals, birds, reptiles, amphibians
• High quality, Reliable and Economical
• Superior stability at 4˚C

Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland in response to stimulation by adrenocortocotropic hormone.… » Read More

Features:

• 30 Minute Assay
• HTS Compatible 96 Well Plate Format
• Works with APIs in HCl. NMP, MeCN, DMF, Ethanol, and Toluene
• Sensitivity 0.082 nM
• Detection with any Fluorescent Plate Reader

Many new synthetic transformations have been developed that use palladium (Pd) compounds for the catalysis of carbon-carbon and carbon-heteroatom coupling reactions such as the Buchwald-Hartwig, Heck, Kumada, Negishi, Nozaki-Hiyama, Sonogashira, Stille, SuzukiMiyaura, and Tsuji-Trost transformations.… » Read More

Now Measure Cortisol from 50 pg/mL – 3200 pg/mL

Cortisol, (hydrocortisone, compound F) is the primary glucocorticoid produced and secreted by the adrenal cortex in humans and other primates. It is often referred to as the “stress hormone” as it is involved in the response to stress, and affects blood pressure, blood sugar levels, and other actions of stress adaptation.… » Read More

Rapid environmental change, including global warming, presents significant challenges in adaptation for all residents of a changing biome.  The ability to adapt to these challenges, to cope with the environmental stresses created by them, is a key factor in determining what species are likely to survive and which might be pushed to extinction.… » Read More

Over activated or over abundant neutrophils generate superoxide anion and other reactive oxidants which can create significant oxidative stress and may play a key role in the progression and development of both acute and chronic inflammatory disease.   Understanding and eventually blocking the burst of neutrophil activity associated with some inflammatory diseases holds promise as a treatment modality for a number of diseases that are currently poorly managed for patients. … » Read More

Acute kidney injury (AKI) is an increasing common clinical disorder affecting more than 20% of patients in hospital.  There are multiple causes for AKI, but one of the most significant is ischemia-reperfusion injury (IRI), characterized by severe renal tubular damage and rapid worsening of renal function. … » Read More

Studies in a wide range of species^1-5,7 have shown that the stress hormone cortisol can be measured in hair samples.  Furthermore, since it has been proven cortisol is deposited in hair as the hair grows, hair cortisol levels can provide information on chronic or long term stress levels that may not be observable in the relative snap-shot provided by fecal or blood samples.  … » Read More

Under normal cellular conditions there is a constant balancing act between the creation of reactive oxygen species (ROS) and their elimination by the antioxidant system including enzymes such as superoxide dismutase, glutathione peroxidase, and catalase.  Abnormally low levels of ROS can impede cellular signaling and some normal intracellular reactions, while overly high levels of ROS create oxidative stress which can lead to the improper oxidation of lipids, proteins and DNA resulting eventually resulting in apoptosis, necrosis, and other cellular damage.… » Read More

When considering the impact of chemicals our planet and the life forms that live here, it is instinctive to focus on things like highly toxic or carcinogenic solvents or other industrial materials. These sorts of toxins certainly are harmful to the environment and appropriate containment and disposal is a must, however there is another group of chemicals that pose significant but much less understood risk – prescription and over the counter drugs. … » Read More

Animal welfare has been defined as “a state of complete health, both physical and mental, in which the animal is in harmony with its environment.   Welfare assessment is an important component in the care of all zoo animals, but is even more critical for individuals who were hand-reared because their reactions to various potential environmental stressors may be different from those of animals reared by a parent. … » Read More

The physical “costs” of reproduction to an organism, particularly under less than ideal conditions, have been the subject of many studies in recent years.  Theories suggest the physical investment in a pregnancy may sometimes come at the cost of either individual survival or future reproductive events.  … » Read More

Thanks to many years of careful planning and habitat restoration, several species including Black footed ferrets, California condors, Grey wolves, Musk ox, Golden eagles and Northern river otters have been or are being reintroduced into areas of their range where historic populations have disappeared or greatly diminished due to pressure from various human activities.  … » Read More

Glomular Filtration Rate (GFR) is the metric normally used to assess kidney function. It can be determined either by measuring the concentration of endogenous markers such as urea nitrogen or creatinine in serum, or by measuring the clearance rate of exogenous indicators such as inulin, iohexol or 51Cr-EDTA or 125I-iothalamate.… » Read More

It has been demonstrated cancer chemotherapy depends on administration of treatment at times that coincide with both optimal drug metabolism and cellular processes such as apoptosis, DNA repair and changes to cell cycle progression. Given we know immune response is also strongly influenced by circadian cycles, it seems reasonable to consider the possibility that the daily cycles of cytokines and immune response proteins may also contribute to the effectiveness of chemotherapeutics. … » Read More

Monitoring of reproductive endocrinology is a very important tool in the management of animal populations both in captivity and in the wild.   Whenever possible measuring key indicators such as estrogens, progestogens, and androgens using non-invasive sample types is prefered so that animals under study are not subjected to the stress of handling and blood collection.… » Read More

Stress and mineralization responses to environmental stress in Mantis Shrimp

Calcified marine organisms face a double edged sword as global warming increases not only the temperature of ocean waters, but also the level of acidification.  Many such organisms that live within the intertidal zone are already adapted to cope with the broad fluctuations in temperature and pH that occur with each tidal cycle – but precisely how the species cope with the stressors of increased temperature and decreased pH varies widely and have costs that can include increased energy demands, growth restriction, physiological stress, and changes in mineralization. … » Read More

Changes in the length of day throughout the year can be a marker for critical species functions such as migration, hibernation, and mating.  These changes have been shown to affect an organism’s morphology and hormones as well as other biomarkers. For example, the thyroid hormones T₃ and T₄ have previously been demonstrated to have roles in the regulation of photoperiod response in birds.  … » Read More

Allopregnanolone is made from progesterone which is converted into 5α-dihydroprogesterone by 5α-reductase type I, and 3α-hydroxysteroid oxidoreductase isoenzymes. This 5α-dihydro intermediate is then converted into allopregnanolone. 3α-hydroxysteroids do not interact with classical intracellular steroid receptors but bind stereoselectively and with high affinity to receptors for the major inhibitory neurotransmitter in brain, γ-amino-butyric acid (GABA). … » Read More

Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role.  Normally stored in azurophilic granules, MPO is released into the extracellular space during degranulation. There, as part of the neutrophils “respiratory burst” it produces hypochlorous acid from hydrogen peroxide and Cl^–.… » Read More

The measurement of stress hormones such as corticosterone or cortisol is generally associated with research focused on impacts of stress or stress management. However, there are many times when it may be just as important to rule out stress as a potential cause for neurological symptoms or behaviors.… » Read More

Stress – the condition keepers and animal husbandry professionals most want to avoid for the animals in their care. Psychosocial and environmental stress can have significant effects not just on day to day mood and demeanor, but also on the immune system, reproduction, and life span.… » Read More

Oxidative stress has been implicated in a wide variety of pathologies including Parkinson’s disease, Alzheimer’s disease, atherosclerosis, heart attack and cardiovascular disease, cancer, diabetes, and chronic fatigue syndrome. Oxidative stress also triggers a process called mitohormesis, which may play a significant role in normal aging.… » Read More

Glutathione (GSH) is a tripeptide (gamma-L-glutamyl-L-cysteinylglycine) that is found in all mammalian tissues and is especially concentrated in the liver (1-10 mM).  Along with its known function combating oxidative stress, GSH is also important for the detoxification of xenobiotics and metabolites, the regulation of cell growth and cell death, the regulation of the nitric oxide cycle, the storage of cysteine, the maintenance of redox potential and the modulation of immune function.… » Read More

An important consideration in the study of hormones is their variability in response to internal and external stimuli, which can provide insight into a variety of biological processes of interest to conservation and management. This essential variability is, of course, what makes these molecules valuable to study, but it adds interesting complications to the sample collection.… » Read More

• ≤ 5 μL Serum or Plasma Sample: for duplicate measurements
• No Extractions Needed
• Validated for Multiple Sample Types: Plasma, Serum, Urine, Fecal Extracts, Media and Dialysates
• High Quality and Economical
• All with superior 4°C Liquid Stability

Metabolomics involves the characterization of small molecule metabolites found in an organism, increasingly used in health applications such as pharmacology, pre-clinical drug trials, toxicology, transplant monitoring, newborn screening and clinical chemistry.… » Read More

Dehydroepiandrosterone (DHEA) is the most abundant circulating C19 steroid in humans. Although DHEA itself has very low androgenic activity, it is the major intermediate in the biosynthesis of the more potent androgen and estrogen sex steroids. DHEA is secreted primarily by the adrenal glands, but may also be produced in the gonads and brain.… » Read More

17-Hydroxyprogesterone (17-HO-P) is a steroid hormone from the androgen group found in mammals, reptiles, birds, and other vertebrates. It was first isolated from the adrenal glands of cattle by Pfiffner and North at Parke, Davis and Company in Detroit, Michigan in 1940¹.… » Read More

Cyclic adenosine monophosphate (cyclic AMP or cAMP) and cyclic guanosine monophosphate (cyclic GMP or cGMP) are prolific intracellular signaling molecules. cAMP is synthesized from adenosine triphosphate (ATP) by the enzyme adenylyl cyclase (AC) and used for intracellular signal transduction in many different organisms.… » Read More

Glucocorticoids like corticosterone guide fundamental processes associated with converting sugar, fat, and protein stores to useable energy, inhibiting swelling and inflammation, and suppressing immune responses following a stress event. Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland in response to stimulation by the adrenocorticotropic hormone.… » Read More

Researchers examining developmental antecedents of depression in adolescents looked at the relationship between cognitive vulnerabilities and poor recovery of the biological stress system as measured through heart rate and cortisol. In their study published in February 2016, authors found a strong link between higher depressive symptoms and the combination of greater cognitive vulnerabilities and lower cortisol and heart rate recovery.… » Read More

The Arbor Assays Prostaglandin E2 (PGE2) Multi-Format EIA Kit uses a unique Prostaglandin E2 monoclonal antibody developed by Arbor Assays that allows one assay to handle all the formats and sensitivities previously covered by the hugely popular and well-published K018 series of PGE2 immunoassays (EIA, CLIA and High-Sensitivity EIA), which it replaces.… » Read More

As climate change and the human population continues to increase, understanding the impact these changes have on our world and the species it harbors becomes ever more critical. Experimental understanding of the stress responses of key animal species can provide a means of quantitatively measuring the biological impact of factors like temperature change, xenobiotic contamination, crowding, and resource loss individually or in combinations that mimic projected real world conditions.… » Read More

The neuropeptides oxytocin and vasopressin were isolated and synthesized by Vincent du Vigneaud at Cornell Medical College in 1953, work for which he received the Nobel Prize in Chemistry in 1955. Oxytocin is highly conserved across vertebrate species and consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail.… » Read More

Levonorgestrel (LNG) is a synthetic steroid analogue used in a number of birth control methods. Levonorgestrel was first made in the 1960s and has been used as a method of birth control since the 1980s. Today it is on the World Health Organization’s List of Essential Medicines, the most important medications needed in a basic health system, and is available as a generic medication.… » Read More

The neuropeptides oxytocin and vasopressin were isolated and synthesized by Vincent du Vigneaud at Cornell Medical College in 1953, work for which he received the Nobel Prize in Chemistry in 1955. The neurohypophysial hormone arginine vasopressin (AVP), also known as antidiuretic hormone, is involved in a wide range of physiological regulatory processes, including renal water reabsorption, cardiovascular homeostasis, hormone secretion from the anterior pituitary, and modulation of social behavior and emotional status.… » Read More

The normalization of specific quantitative assay results is critical to interpretation of biological system status. With cells grown in culture and lysed, a simple total protein determination such as the Bradford assay developed by Marion Bradford at the University of Georgia in 1976 will give an estimate of cellular protein.… » Read More

Endothelin-1 (ET-1), a peptide of 21 amino acid residues, is a pleiotropic molecule known for its action as a potent vasoconstrictor. ET-1 is one of a family of three proteins encoded by distinct genes that also includes Endothelin-2 (ET-2) and Endothelin-3 (ET-3).… » Read More

The World Health Organization has estimated 250 million children have moderate to severe Vitamin A deficiency (VAD) due to lack of adequate nutrition, and the rising cost of food staples around the world further exacerbates this problem. In addition to nutritional deficiencies, infectious stresses have been shown to depress retinol concentrations causing individuals with diseases that interfere with adsorption or storage of vitamin A such as cystic fibrosis, pancreatic insufficiency, duodenal bypass, celiac, cirrhosis etc.… » Read More

• SENSITIVE: Measure < 500 fmol Free Thiol
• RAPID: Bright Fluorescence in Seconds
• SOLUTION STABILITY: 2 Months at 4°C
• LARGE STOKES SHIFT: Excite at 390 nm, Read at 510 nm
• LOW BACKGROUND: Almost No Signal from Unreacted ThioStar®
• VERSATILE: Soluble in Common Solvents and Water
• FLEXIBLE FORMAT: Microplates, Cuvettes or HPLC

Simple, Stable and Sensitive

The ThioStar^® molecule is designed to quantitatively measure thiol groups generated or present in samples.… » Read More

The hugely popular K018 series of PGE₂ Immunoassays, sold since 2010, has been discontinued as of June 1, 2015, and replaced with K051-H, PGE₂ Multi-Format EIA Kit, for measurement of PGE₂.  The 2014 launched DetectX^® PGE₂ EIA kit, K051-H1 and K051-H5, uses an internally developed monoclonal to PGE₂. … » Read More

NOVEMBER 16, 2015 – Ann Arbor SPARK recently presented its annual FastTrack award to Arbor Assays for its impressive record of growth, along with 13 other Washtenaw County-based companies.

FastTrack awards are presented to companies with a 2011 revenue of at least $100,000 and an annual growth of 20 percent during the subsequent three years, which Arbor Assays exceeded.… » Read More

The PGE2 Multi-format EIA, K051-H1/H5, takes all of the best features of our previous three PGE2 kits and combines them into one. It is a fabulous product! If you’ve been a long time K018 user, no need to fret. We are offering customers who have used that kit in the past a free K051 kit to try, with your next order.… » Read More

Arbor Assays and the International Society of Wildlife Endocrinology (ISWE) signed an exclusive agreement for Arbor Assays to supply ISWE members with EIA kits for wildlife conservation research.  The Agreement was signed this October at SEZARC, the South-East Zoo Alliance for Reproduction & Conservation in Yulee, Florida.… » Read More

The BEST Internship Pilot is a program offered by the NIH to doctoral students seeking internships in six career areas- law, business, communication, teaching, community engagement, and government. Broadening Experiences in Scientific Training (BEST) was designed for doctoral students interested in exploring careers beyond academia.… » Read More

The DetectX® Aldosterone CLIA assay is the most sensitive assay available anywhere to measure Aldosterone.  Sensitivity of 2.06 pg/mL. Multi-species.  Perfect complement to our colorimetric Aldosterone EIA kit, K052-H1 and K052-H5.

New monoclonal-based DetectX® Multi-Format Prostaglandin E2 kit, K051-H1 and K051-H5, measures PGE2 in cell lysates, serum, plasma, tissue, urine and media without extraction.  Multi-format kit allows measurement of PGE2 from 1,000 down to 2 pg/mL.

 … » Read More

The DetectX® Oxytocin CLIA assay is the world’s most sensitive assay to measure oxytocin.  Sensitivity of 6.3 pg/mL.  Measures all species.

The DetectX^® Serum Estradiol Immunoassay (EIA/ELISA) kit is designed to quantitatively measure 17ß-estradiol present in serum and plasma samples.

Read More >>

Another unique product.

The DetectX^® Allopregnanolone Immunoassay (EIA/ELISA) kit is designed to quantitatively measure Allopregnanolone present in serum, plasma, extracted dried fecal samples, urine and tissue culture media samples.

Read More >>

An allopregnanolone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve.… » Read More

On June 5th, 2013 we hosted an Open House to celebrate our recent expansion and renovation.  We doubled our premises and added improved workspace, workflow, lighting, energy efficiency, and installation of a larger generator.  We hosted 50 local business leaders, bankers, attorneys, web designers, artists and the people who made the expansion possible.… » Read More

For the sixth consecutive year as Arbor Assays, we, along with family and friends, have participated in Walk MS on June 22, 2013 to raise awareness and funds to support the National Multiple Sclerosis Society.  The weather was hot, the Hudson Mills Metropark route relaxing and we had a great time!  … » Read More

Reactive oxygen species (ROS) are produced as a consequence of normal aerobic metabolism and the free radicals produced may be responsible for a variety of pathological conditions including Parkinson’s and Alzheimer’s diseases, atherosclerosis, myocardial infarction, and chronic fatigue syndrome.

The FRAP™ (Ferric Reducing Ability of Plasma) Assay Kit is designed to quantitatively measure antioxidant status in a variety of samples.… » Read More

Our new Galactose Colorimetric Detection kit allows galactose to be easily and conveniently measured in serum, plasma or buffer solutions.

Our Newsletter E mail sign up form is on every web page.  Remember – your information is secure and we never share your E mail or contact information.

High Sensitivity PGE2 EIA Kit –

“In vitro enantioselective pharmacodynamics of Carprofen and Flunixin-meglumine in feedlot cattle”

Link: http://onlinelibrary.wiley.com/doi/10.1111/jvp.12052/abstract;jsessionid=F68488B7FBF2E8F8F55C1CA21377035B… » Read More

Cyclic AMP Chemiluminescent CLIA Kit –

“Autocrine Regulation of Macrophage Activation via Exocytosis of ATP and Activation of P2Y11 Receptor”

Link: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0059778

Urea Nitrogen (BUN) Detection Kit –

“Associations between endotoxin-induced metabolic changes and temperament in Brahman bulls”

Link: http://onlinelibrary.wiley.com/doi/10.1111/jpn.12074/abstract?deniedAccessCustomisedMessage=&userIsAuthenticated=false

Corticosterone Colorimetric EIA Kit –

“Effect of Breed, Cage Type, and Reproductive Phase on Fecal Corticosterone Levels in Doe Rabbits“

Link: http://www.tandfonline.com/doi/abs/10.1080/10888705.2013.768903… » Read More

Just add samples!  Our new Total Protein Detection kit gives you everything you need to measure the protein content of serum, urine, lysates or homogenates.  The most sensitive BCA kit available can measure <1.7 ug/mL of protein or about 84 pg of protein!  … » Read More

Formaldehyde Fluorescent Kit – “GROWTH AND DEVELOPMENT SYMPOSIUM:Development, characterization, and use of a porcine epiblast-derived liver stem cell line: ARS-PICM-19″

Link: http://www.journalofanimalscience.org/content/91/1/66.abstract?sid=be28c1d4-618c-4bb6-986b-a96d19aa72ad

ThioStar® – “Transcription and redox enzyme activities: comparison of equilibrium and disequilibrium levels in the three-spined stickleback”

Link: http://rspb.royalsocietypublishing.org/content/280/1755/20122974.short… » Read More

J. Drug Targeting, 11 December 2012,  “Preparation, characterization, in vivo and biochemical evaluation of brain targeted Piperine solid lipid nanoparticles in an experimentally induced Alzheimer’s disease model” by M. Yusef, et al.

Link http://informahealthcare.com/doi/abs/10.3109/1061186X.2012.747529

Cyclic GMP EIA – “Distant effects of nitric oxide inhalation in lavage-induced lung injury in anaesthetised pigs”

Link: http://www.ncbi.nlm.nih.gov/pubmed/23198970

PGE2 EIA – “Cyclooxygenase-2 Is a Target of MicroRNA-16 in Human Hepatoma Cells”

Link: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0050935

PGE2 EIA – “Ethanolic Leaf Extract of Croton Zambesicus (MÜll.… » Read More

Urinary Creatinine Paper -“Reversal of the deleterious effects of chronic dietary HFCS-55 intake by PPAR-δ agonism correlates with impaired NLRP3 inflammasome activation?” 

Link: http://www.sciencedirect.com/science/article/pii/S0006295212006909

Serum Creatinine Paper – “Stem Cell Transplantation Increases Antioxidant Effects in Diabetic Mice”

Link: http://www.biolsci.org/v08p1335.htm

Cortisol EIA Paper– “Yeast cell wall supplementation alters the performance and health of newly received crossbred heifers”

Link: http://repositories.tdl.org/ttu-ir/bitstream/handle/2346/46725/YOUNG-THESIS.pdf?sequence=1… » Read More

The calmodulin/calcium-activated K⁺ channel KCa3.1 is expressed in red and white blood cells, epithelia and endothelia, and possibly central and peripheral neurons. The knowledge about its contribution to neurological functions and behavior is incomplete. The paper examines whether genetic deficiency or pharmacological activation of KCa3.1 change behavior and cerebral monoamine levels in mice.… » Read More

Our new Glucose Fluorescent Detection kit, K039-F1, for serum, plasma, urine, cell extract samples has been launched.  The 2 by 96-well plate contains everything needed to measure glucose in < µL amounts of serum and plasma.  All liquid, 4°C stable kit.… » Read More

The paper “Effects of lipoic acid on lipolysis in 3T3-L1 adipocytes” was published in the Journal of Lipid Research today.  This paper describes the use of our Direct Cyclic AMP EIA kit, K019-H1, to measure this analyte in 3T3-L1 adipocytes.

Read More: http://www.jlr.org/content/early/2012/08/31/jlr.M027086.abstract… » Read More

A paper in PLOS One, entitled “(S)-α-Chlorohydrin Inhibits Protein Tyrosine Phosphorylation through Blocking Cyclic AMP – Protein Kinase A Pathway in Spermatozoa” was published today.  This paper describes the use of our chemiluminescent cAMP assay, K019-C1, in the measurement of cAMP in rat sperm.… » Read More

We have just received our new kit boxes.  It is a brighter rethink of our original kit box made from a more water resistant card stock. Still completely recyclable.  Look for these in your lab soon.

Arbor Assays has awarded a €1,000.00 travel grant to Ekaterina Pavlova of the A. N. Severtsov Institute of Ecology and Evolution in Moscow, Russia to help offset costs associated with her presentation at the 3rd ISWE Conference to be held in Vienna, Austria, September 23-26, 2012.  … » Read More

For the non-invasive determination of E1S and metabolites in serum, plasma, urine and fecal samples we have launched our new Estrone-3-Sulfate (E1S) EIA Assay Kits, K038-H1 and K038-H5. Clinical studies have indicated the utility of measuring estrone-3-sulfate (E1S) in samples from male horses to monitor cryptorchidism.… » Read More

Arbor Assays announces the launch of its 2 by 96-well plate Hydrogen Peroxide Colorimetric Detection Kit, K034-H1 to supplement its very popular super-sensitive Fluorescent H₂O₂ Detection kit, K034-F1. The kit can measure from 100 to 3.125 µM H₂O₂ in 15 minutes using 4°C stable all-liquid components.… » Read More

For the non-invasive determination of PDG and its metabolites in urine and fecal samples, or in extracted serum and plasma we have launched our new Pregnanediol-3-Glucuronide (PDG) EIA Assay Kits, K037-H1 and K037-H5. Clinical studies have indicated the utility of measuring PDG in samples of urine to monitor ovarian function in females

Kit manual:  Pregnanediol-3-Glucuronide (PDG) EIA Assay Kits

The world’s most sensitive EIA kit for measuring human Osteopontin (OPN) has been launched. This is a double monoclonal antibody based kit where the antibody coated on the plate recognizes only intact hOPN.  The assay which can be used for EDTA plasma, urine, milk and culture media, shows linear response with dilution and will measure down to 250 pg/mL hOPN.… » Read More

For the non-invasive determination of E1G and its metabolites in urine and fecal samples, or in extracted serum and plasma we have launched our new Estrone-3-Glucuronide (E1G) EIA Assay Kits, K036-H1 and K036-H5. Clinical studies have indicated the utility of measuring estrone-3-glucuronide (E1G) in samples of urine to monitor ovarian function in females

Kit manual:  Estrone-3-Glucuronide (E1G) EIA Assay Kits

For the non-invasive determination of pregnancy in urine samples we have launched our new Ceruloplasmin (Cp) Colorimetric Activity Assay Kit, K035-H1.  This kit has been shown to measure increasing levels of Cp activity which is expressed in elevated amounts during pregnancy.… » Read More

Visit us at booth # 722 at ASCB, San Diego, December 5-9, 2009
We will have an exhibit booth at the American Society for Cell Biology for the first time. Our booth number is 722 so please stop by and visit us.… » Read More

Arbor Assays announces new distributor for South East Asia

Arbor Assays announces today that it has signed 3 new distributors in SE Asia. vCell Science is out new distributor in Singapore and Axon Scientific will represent us in Malaysia. We have also signed PT Genetika in Indonesia.… » Read More

Arbor Assays announces new distributor for Spain and Portugal
Arbor Assays today announced the appointment of exclusive distributor for Spain and Madrid. LabNet Biotécnica S.L. has been appointed as Arbor Assays’ exclusive distributor in Spain and Portugal. Full contact details can be found in our Distributor section.… » Read More

Arbor Assays announces the addition of 3 new distributors
Arbor Assays today announced the appointment of exclusive distributors for South Africa and Turkey and an additional distributor in China. BIOCOM Biotech has been appointed as Arbor Assays’ exclusive distributor in South Africa.… » Read More

May 2009 Edition of Clinical Chemistry, 55:895-903

Two Arbor Assays scientists are co-authors on a paper that has just been published in the May 2009 edition of Clinical Chemistry. The paper is entitled “New Dual Monoclonal ELISA for Measuring Plasma Osteopontin as a Biomarker Associated with Survival in Prostate Cancer: Clinical Validation and Comparison of Multiple ELISAs” by Peter H.… » Read More

ANN Arbor, Mich. — (BUSINESS WIRE)
February 25, 2009

January 14, 2009: Arbor Assays and University of Michigan Patent New Demethylase Assay Technology

Two scientists at Arbor Assays, LLC and Professor Ray Trievel from the Department of Biological Chemistry at the University of Michigan filed a US patent application that relates to a new, novel method to detect demethylase enzyme activity.… » Read More

Arbor Assays receives Bronze Team Award for the 2008 MS Walk from the Michigan chapter of the MS Society

Arbor Assays LLC has received a Bronze Team Award from the Michigan chapter of the MS Society for its money raising activity.… » Read More

Cortisone was identified by Mason, Myers and Kendall in 1936 as Compound E extracted from bovine suprarenal gland tissue that had the qualitative but not quantitative activity of cortin. The presence of multiple cortin-like compounds led the authors to speculate that the study of Compound E would reveal the nature of cortin.… » Read More

Glomular Filtration Rate (GFR) is the metric normally used to assess kidney function. It can be determined either by measuring the concentration of endogenous markers such as urea nitrogen or creatinine in serum, or by measuring the clearance rate of exogenous indicators such as inulin, iohexol or 51Cr-EDTA or 125I-iothalamate.… » Read More

Features:

• 30 Minute Assay
• HTS Compatible 96 Well Plate Format
• Works with APIs in HCl. NMP, MeCN, DMF, Ethanol, and Toluene
• Sensitivity 0.082 nM
• Detection with any Fluorescent Plate Reader

Many new synthetic transformations have been developed that use palladium (Pd) compounds for the catalysis of carbon-carbon and carbon-heteroatom coupling reactions such as the Buchwald-Hartwig, Heck, Kumada, Negishi, Nozaki-Hiyama, Sonogashira, Stille, SuzukiMiyaura, and Tsuji-Trost transformations.… » Read More

Now Available

20-Hydroxyecdysone EIA Kit, K066-H

FEATURES:

Use: Measure 20-hydroxyecdysone from a variety of species
Broad Range: 25000 pg/mL – 100 pg/mL
Time to Answer: 2.5 hours
Sample Volume: 50 μL
Specificity: Highly Specific to 20-hydroxyecdysone, very low cross reactivity with other steroids
Ecdysones are a group of steroid hormones that regulate metamorphosis, cell death, and reproduction in arthropods.… » Read More

Neurosteroids have the ability to rapidly alter neuronal excitability through interactions with ligand-gated ion channels and cell surface receptors.  Among these neurosteroids, pregnenolone, progesterone, allopregnanolone, cortisol, and dehydroepiandrosterone are known to be involved in biological functions that have been implicated in the pathophysiology of various psychiatric disorders.… » Read More

The World Health Organization has estimated 250 million children have moderate to severe Vitamin A deficiency (VAD) due to lack of adequate nutrition, and the rising cost of food staples around the world further exacerbates this problem. In addition to nutritional deficiencies, infectious stresses have been shown to depress retinol concentrations causing individuals with diseases that interfere with adsorption or storage of vitamin A such as cystic fibrosis, pancreatic insufficiency, duodenal bypass, celiac, cirrhosis etc.… » Read More

Cranberry juice has long been known to have antioxidant properties attributed to high levels of polyphenols.  While much research has been done concerning the impact of cranberries and cranberry juice on urinary tract infections, fewer studies have examined their effects on other pathologies.  … » Read More