Cyclic AMP Direct EIA Kit

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  • USE - Measure cAMP in 2.5 Hours
  • SAMPLE - Lysates, Urine, Plasma, Saliva, Tissue
  • SAMPLES/KIT - 39 or 231 in Duplicate
  • SENSITIVITY - Measure < 5 fmol cAMP in sample
  • STABILITY - Stable 4˚C Liquid Reagents

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The DetectX® Cyclic AMP (cAMP) Direct Immunoassay kit is designed to quantitatively measure cAMP present in lysed cells, tissue, plasma, urine, saliva and culture media samples. The kit is unique in that all samples and standards are diluted into an acidic Sample Diluent which is designed to lyse cells, inactivate PDE enzymes, and stabilize cAMP.  A cAMP standard is provided to generate a standard curve for the assay. A special Plate Primer is added to all the wells and standards or samples in Sample Diluent are pipetted into the primed clear microtiter plate. A cAMP-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of an antibody to cAMP. After 2 hours, the plate is washed and substrate is added. The substrate reacts with the bound cAMP-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is read. An optional Acetylation Format allows super low concentrations of cAMP to be measured.

Adenosine-3’,5’-cyclic monophosphate, or cyclic AMP (cAMP), is one of the most important second messengers and a key intracellular regulator. Discovered by Sutherland and Rall in 1957, it functions as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Adenylate cyclase is activated by the hormones glucagon and adrenaline and by G protein. Liver adenylate cyclase responds more strongly to glucagon, and muscle adenylate cyclase responds more strongly to adrenaline. cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterase. In the Human Metabolome Database there are 166 metabolic enzymes listed that convert cAMP.

Cyclic AMP EIA Standard Curve

Data Reduction Template by MyAssays

Item Catalog # Price
Cyclic AMP Direct EIA Kit, 1 PlateK019-H1$295.00Inquire About This Product
Cyclic AMP Direct EIA Kit, 5 PlateK019-H5$1,150.00Inquire About This Product


Date Publication
Jun 19 2017 Regulation of type III secretion of translocon and effector proteins by the EsaB/EsaL/EsaM complex in Edwardsiella tarda
May 30 2017 Attenuated diuresis and natriuresis in response to glucagon-like peptide-1 in hypertensive rats are associated with lower expression of the glucagon-like peptide-1 receptor in the renal vasculature
May 03 2017 Ixazomib enhances parathyroid hormone (PTH)-induced β-catenin/T-cell factor (TCF) signaling by dissociating β-catenin from the PTH receptor
May 01 2017 Characterizing New Photoreceptors to Expand the Optogenetic Toolbox
Mar 07 2017 Insulin Sensitizer Prevents and Ameliorates Experimental Type-1 Diabetes
Feb 23 2017 Levels of cyclic AMP and cyclic GMP in porcine oocyte cumulus complexes and cumulus free oocytes derived from small and middle follicles during the first 24 hour period of in vitro maturation
Feb 14 2017 Development and Application of Human Renal Proximal Tubule Epithelial Cells for Assessment of Compound Toxicity
Jan 20 2017 Bicarbonate‐sensing soluble adenylyl cyclase is present in the cell cytoplasm and nucleus of multiple shark tissues
Jan 05 2017 Human LH and hCG stimulate differently the early signalling pathways but result in equal testosterone synthesis in mouse Leydig cells in vitro
Dec 09 2016 Butyrate attenuates lipolysis in adipocytes co-cultured with macrophages through non-prostaglandin E2–mediated and prostaglandin E2–mediated pathways
Nov 18 2016 Induction of Airway Allergic Inflammation by Hypothiocyanite via Epithelial Cells
Oct 05 2016 Induction of peptidylarginine deiminase 2 and 3 by dibutyryl cAMP via cAMP-PKA signaling in human astrocytoma U-251MG cells
Aug 13 2016 New insights into selective PDE4D inhibitors: 3-(Cyclopentyloxy)-4-methoxybenzaldehyde O-(2-(2,6-dimethylmorpholino)-2-oxoethyl) oxime (GEBR-7b) structural development and promising activities to restore memory impairment
Aug 10 2016 Angiotensin II counteracts the effects of cAMP/PKA on NHE3 activity and phosphorylation in proximal tubule cells
Jun 22 2016 Differential impact of acute and prolonged cAMP agonist exposure on protein kinase A activation and human myometrium contractile activity
May 25 2016 Immunogenicity and protective efficacy against ETEC colonization following intradermal, sublingual or oral vaccination with EtpA adhesin
May 09 2016 Blood Group O–Dependent Cellular Responses to Cholera Toxin: Parallel Clinical and Epidemiological Links to Severe Cholera
Apr 14 2016 Overcoming Enterotoxigenic Escherichia coli Pathogen Diversity: Translational Molecular Approaches to Inform Vaccine Design
Jan 01 2016 Effects of α-lipoic acid on lipid metabolism and mitochondrial biogenesis in adipocytes: study of the molecular mechanisms involved
Dec 12 2015 Follicle-stimulating hormone potentiates the steroidogenic activity of chorionic gonadotropin and the anti-apoptotic activity of luteinizing hormone in human granulosa-lutein cells in vitro
Dec 01 2015 Identification of a Novel Protein Kinase A Inhibitor by Bioluminescence-Based Screening
Nov 17 2015 H295R expression of melanocortin 2 receptor accessory protein (MRAP) results in ACTH responsiveness
Oct 30 2015 Allosteric modulation of metabotropic glutamate receptor 4 activates IDO1-dependent, immunoregulatory signaling in dendritic cells
Oct 12 2015 Edwardsiella tarda EscE (Orf13 protein) is a type III secretion system secreted protein that is required for the injection of effectors, secretion of translocators, and pathogenesis in fish
Jun 18 2015 Ecklonia cava Polyphenol Has a Protective Effect against Ethanol-Induced Liver Injury in a Cyclic AMP-Dependent Manner
Jun 11 2015 Molecular and Cellular Effects Induced in Mytilus galloprovincialis Treated with Oxytetracycline at Different Temperatures
Jun 10 2015 Growth on glucose decreases cAMP-CRP activity while paradoxically increasing intracellular cAMP in the light-organ symbiont Vibrio fischeri
May 19 2015 Prenatal ethanol exposure and placental hCG and IGF2 expression
Apr 29 2015 Host cell type-dependent translocation and PhoP-mediated positive regulation of the effector SseK1 of Salmonella enterica
Apr 16 2015 Synthesis, biological activities and pharmacokinetic properties of new fluorinated derivatives of selective PDE4D inhibitors
Apr 14 2015 Dual signal transduction pathways activated by TSH receptors in rat primary tanycyte cultures
Mar 23 2015 Evaluating the role of hnRNP-C and FMRP in the cAMP-induced APP metabolism
Feb 27 2015 Association of arginine vasopressin surrogate marker urinary copeptin with severity of autosomal dominant polycystic kidney disease (ADPKD)
Feb 26 2015 Regulation of blood pressure, oxidative stress and AT1R by high salt diet in mutant human dopamine D5 receptor transgenic mice
Feb 09 2015 Identification and functional characterization of a novel Edwardsiella tarda effector EseJ
Feb 05 2015 Renal nerve stimulation leads to the activation of the Na+/H+ exchanger isoform 3 via angiotensin II type I receptor
Dec 17 2014 Activated astrocytes enhance the dopaminergic differentiation of stem cells and promote brain repair through ​bFGF
Nov 03 2014 Regulation of the ahpC Gene Encoding Alkyl Hydroperoxide Reductase in Mycobacterium smegmatis
Oct 23 2014 Exploitation of Cholane Scaffold for the Discovery of Potent and Selective Farnesoid X Receptor (FXR) and G-Protein Coupled Bile Acid Receptor 1 (GP-BAR1) Ligands
Oct 01 2014 Cardiac Control in the Pacific Hagfish (Eptatretus Stoutii)
Oct 01 2014 Discovery of Novel Adenylyl Cyclase Inhibitor by Cell-Based Screening
Aug 20 2014 Restoring multidrug-resistance associated protein 3 attenuates cell proliferation in polycystic kidney
Mar 05 2014 Cyclic Nucleotide (cAMP and cGMP) Assays and Capture ELISA for Quantitative Analysis of Plasmodium falciparum Blood-stage Egress
Jan 14 2014 Role of Extracellular Signal-Regulated Kinase 5 in Adipocyte Signaling
Dec 07 2013 An exploratory investigation of various modes of action and potential adverse outcomes of fluoxetine in marine mussels
Nov 18 2013 Enterotoxigenic Escherichia coli secrete a highly conserved mucin-degrading metalloprotease to effectively engage intestinal epithelial cells
Jul 29 2013 A primary culture system of mouse thick ascending limb cells with preserved function and uromodulin processing
Jun 26 2013 Activation of Nucleotide-Oligomerization Domain Containing Protein 1 Induces Lipolysis Through NF-κB and the Lipolytic PKA Activation in 3T3-L1 Adipocytes
Apr 12 2013 Cyclic-AMP Mediated Regulation of ABCB mRNA Expression in Mussel Haemocytes
Apr 09 2013 Phosphodiesterase 7 inhibitor reduced cognitive impairment and pathological hallmarks in a mouse model of Alzheimer's disease
Jan 16 2013 Zolmitriptan: A Novel Portal Hypotensive Agent Which Synergizes with Propranolol in Lowering Portal Pressure
Nov 30 2012 cAMP/Protein Kinase A Activates Cystic Fibrosis Transmembrane Conductance Regulator for ATP Release from Rat Skeletal Muscle during Low pH or Contractions
Nov 14 2012 Sorting nexin 1 loss results in D5 dopamine receptor dysfunction in human renal proximal tubule cells and hypertension in mice
Sep 10 2012 Identifying Target Genes related to Respiratory Network Dysfunction in a Mouse Model for the Rett Syndrome
Aug 14 2012 Effects of lipoic acid on lipolysis in 3T3-L1 adipocytes
Aug 13 2012 Identification of Peptide Lv, a Novel Putative Neuropeptide That Regulates the Expression of L-Type Voltage-Gated Calcium Channels in Photoreceptors

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