PGE2 Multi-Format EIA Kit

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Features

  • USE - Measure PGE2 between 1,000 - 2 pg/mL
  • SAMPLE - Serum, Plasma, Saliva, Urine and TCM
  • CONVENIENT - One kit for rapid, sensitive, low sample volumes
  • SAMPLES/KIT - up to 39 or 231 in Duplicate
  • SENSITIVE - Measure < 0.2 pg per Sample
  • VERSATILE - Works directly with Mouse and Rat Serum

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Multi Species and Most Sensitive

Description

The DetectX Prostaglandin E2 (PGE2) Multi-Format Enzyme Immunoassay kit is designed to quantitatively measure PGE2 present in serum, plasma, urine, tissues, saliva and culture media samples.  This one EIA kit allows all samples to be measured with just one kit.  The monoclonal antibody has super-fast kinetics and shows the same binding characteristics at 2 or 16 hour incubation.  The kit allows samples as low as 2 pg/mL to be measured in less than 3 hours. It exhibits excellent cross reactivity and low sample requirements.

A PGE2 standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A PGE2-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to PGE2. After either an incubation the plate is washed and substrate is added. The substrate reacts with the bound PGE2-peroxidase conjugate. After a 30 minute incubation the intensity of the generated color is read at 450nm.

The new PGE2 monoclonal (3H10) developed to use in the new PGE2 EIA kits has some truly amazing properties:

  • Super Fast Kinetics – Same Assay Range at 2 or 16 hours
  • Incredible Sensitivity – Lowest Standard < 2 pg/mL
  • Superior Cross Reactivity to Other Eicosanoids
  • Super Low Sample Volume Requirements
  • Choice of Incubation Time, Sensitivity, and Sample Volume in a Single Kit

B0Graph

Data Reduction Template by MyAssays

Item Catalog # Price
PGE2 Multi-Format EIA Kit, 1 PlateK051-H1$300.00Inquire About This Product
PGE2 Multi-Format EIA Kit, 5 PlateK051-H5$1,200.00Inquire About This Product


Publications

Date Publication
May 27 2017 AMPK and SIRT1 activation contribute to inhibition of neuroinflammation by thymoquinone in BV2 microglia
May 19 2017 Molecular species of prostaglandins involved in modulating luteinizing hormone pulses of female rats under infectious stress conditions
Apr 14 2017 Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
Mar 10 2017 EP2 receptor antagonism reduces peripheral and central hyperalgesia in a preclinical mouse model of endometriosis
Jan 31 2017 Antibody-mediated depletion of immunosuppressive factors from ovarian carcinoma-associated ascites for investigation of paracrine versus autocrine effects
Nov 16 2016 Cell Expansion-Dependent Inflammatory and Metabolic Profile of Human Bone Marrow Mesenchymal Stem Cells
Sep 22 2016 Novel celecoxib analogues inhibit glial production of prostaglandin E2, nitric oxide, and oxygen radicals reverting the neuroinflammatory responses induced by misfolded prion protein fragment 90-231 or lipopolysaccharide
Sep 20 2016 Human iNKT Cells Promote Protective Inflammation by Inducing Oscillating Purinergic Signaling in Monocyte-Derived DCs
Sep 14 2016 Apple peel polyphenols: A key player in the prevention and treatment of experimental inflammatory bowel disease
Sep 12 2016 Effects of a Novel Dietary Supplement on Indices of Muscle Injury and Articular GAG Release in Horses
Aug 11 2016 Serum amyloid A (SAA) is an early biomarker of influenza virus disease in BALB/c, C57BL/2, Swiss-Webster, and DBA.2 mice
Jun 15 2016 Anti-inflammatory Chitosan/Poly-γ-glutamic acid nanoparticles control inflammation while remodeling extracellular matrix in degenerated intervertebral disc
Jun 15 2016 Cyclooxygenase-2 expression in hepatocytes attenuates non-alcoholic steatohepatitis and liver fibrosis in mice
May 24 2016 Synthesis and reception of prostaglandins in corpora lutea of domestic cat and lynx
May 05 2016 Compositions for Use in Cartilage Breakdown
Apr 04 2016 A Novel Biological Role of α-Mangostin in Modulating Inflammatory Response Through the Activation of SIRT-1 Signaling Pathway
Apr 01 2016 Effects of Aspirin on metabolic alterations in Type 2 Diabetic Rats
Feb 06 2016 Tricyclic pyrazoles. Part 8. Synthesis, biological evaluation and modelling of tricyclic pyrazole carboxamides as potential CB2 receptor ligands with antagonist/inverse agonist properties
Jan 27 2016 Analgesic exposure in pregnant rats affects fetal germ cell development with inter-generational reproductive consequences
Jan 01 2016 Effects of α-lipoic acid on lipid metabolism and mitochondrial biogenesis in adipocytes: study of the molecular mechanisms involved
Dec 01 2015 Functional Characterization of Purinergic Receptors in the Differentiation and Activation of Macrophages
Nov 25 2015 Antimalarial Drug Artemether Inhibits Neuroinflammation in BV2 Microglia Through Nrf2-Dependent Mechanisms
Jul 14 2015 Pomegranate inhibits neuroinflammation and amyloidogenesis in IL-1β stimulated SK-N-SH cells
Mar 25 2015 Quercetin attenuates cyclooxygenase-2 expression in response to acute ureteral obstruction

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