Rare Reagent Generation
For many immunoassays the design of a suitable antigenic molecule is critical to the ability of the assay to specifically and sensitively detect the molecule of interest. The scientists at Arbor Assays are specialized in the design of peptides and organic derivatives to allow for the generation of high quality polyclonal and monoclonal antibodies as well as suitable enzyme and luminescent detection conjugates for immunoassay use.
We maintain relationships with a number of high quality, chemical organic synthesis companies that, under our direction, will efficiently make derivatives of biological molecules suitable for effective conjugation. After reviewing the chemical data on the derivative or peptide to be used for conjugations we will carry out protein labeling utilizing porcine thyroglobulin (PTG) for raising antibodies and BSA for ELISA titering. We prefer using PTG rather than KLH as a carrier protein because of its solubility and size.
PTG can be dissolved in aqueous buffers at 20 mg/mL (far in excess of KLH) and is comprised of 4 subunits each of about 165kDa in size. This solubility can help in estimating incorporation ratios of antigen to PTG spectrophotometrically.
For derivatives containing a free thiol group we will pre-treat the PTG with a reagent to add maleimide groups. For those derivatives with a free carboxylic acid we can chemically activate them using DCC/NHS for coupling to protein lysine groups. PTG and BSA labeled with high levels of antigen are purified on desalting columns, followed by analysis to estimate labeling efficiencies when possible.
Please contact us at contracts@ArborAssays.com for our input into your project.