- Multi Species
- Sample - Urine or Extracted Fecal, Serum, and Plasma Samples
- Samples/Kit - 39 or 231 in Duplicate
- Time to Answer - 2.5 Hours
- Sensitivity - 1.85 pg/mL
- Assay Precision - Intra: 4.6–6.8% CV; Inter: 6.5–8.2% CV
- Stability - 4° Stable Reagents
- Cross Reactivity - Low cross reactivity with other androgens
The DetectX® 11-Ketotestosterone Immunoassay Kit uses a specifically generated antibody to measure 11-ketotestosterone in urine or in extracted fecal, serum, and plasma samples.
Androgenic hormones, such as testosterone, affect the growth, size, and reproduction of many male organisms. In teleost fish, along with testosterone, 11-ketotestosterone plays a significant role by inducing reproductive characteristics in both males and females. In certain male fish, 11-ketotestosterone levels increase during spermatogenesis in spawning season, while in some female fish, 11-ketotestosterone increases prior to yolk deposition to regulate ovarian development.
The presence and involvement of 11-ketotestosterone in other species such as humans have only recently been established. In contrast to fish, primate serum 11-ketotestosterone concentrations were not significantly different in male and female, despite males having significantly higher circulating testosterone. This suggests that 11-ketotestosterone production in these species may not be testis-dependent and primarily originates from adrenal-derived 11-oxyandrogen precursors. Recent studies have discovered there is more 11-ketotestosterone than its precursors, androstenedione and testosterone, in prepubertal children and postmenopausal women compared to men. This highlights the potential use of 11-ketotestosterone as a clinical biomarker to screen adrenal androgen excess in disease conditions such as polycystic ovary syndrome (PCOS) and hirsutism in women.