- Most Sensitive
- Multi Species
- USE - Measure AChE Activity in 20 Minutes or Kinetically
- SAMPLE - CSF, Serum, Plasma, RBC Ghosts
- SAMPLES/KIT - 90 in Duplicate
- SENSITIVE - Measure < 0.25mU/mL of AChE Activity
- STABILITY - All Reagents Stable at 4°C
The DetectX® Acetylcholinesterase (AChE) Activity kit is designed to quantitatively measure AChE activity in a variety of samples. A human AChE standard is provided to generate a standard curve for the assay. The kit utilizes a proprietary non-fluorescent molecule, ThioStar®, that covalently binds to the thiol product of the reaction between the AChE Substrate and AChE in the standards or samples, yielding a fluorescent product read at 510 nm in a fluorescent plate reader with excitation at 390 nm.
Acetylcholinesterases (AChE) appear critical to both development and function of the nervous system. The use of AChE inhibitors as therapeutic agents and pesticides has spurred detailed investigations of cholinesterases since their identification by Dale. Acetylcholine (ACh) is an essential neurotransmitter in the central and peripheral nervous systems. In the brain multiple areas exist where cholinergic neurons are concentrated. Nicotinic and muscarinic ACh receptors are recognized as binding and effector proteins to mediate chemical neurotransmission at neurons, ganglia, heart and smooth muscle fibers and glands. This traditional view of AChE acting solely as neurotransmitter has to be revised based on the findings published both early and late in the last century, demonstrating the non-neuronal cholinergic system.