• Catalog Number K015-F
  • Assay Type Activity Assay
  • Sample Types Serum, Plasma (EDTA and Heparin), RBC Ghosts, Extracted Tissue Sample, Erythrocyte Membranes
  • Sensitivity 0.228 mU/mL
  • Species Species Independent
  • Assay Duration 20 Minutes
  • Samples/Plate 42 in Duplicate
  • Readout Fluorescent, 510 nm Emission / 390 nm Excitation


Assay Principle:

The DetectX® Acetylcholinesterase (AChE) Fluorescent Activity Kit offers a reliable method for quantitatively measuring AChE activity. Compatible sample types include serum, plasma (EDTA and Heparin), RBC ghosts, and extracted tissue samples. This activity assay features a 20-minute run time, providing efficient and accurate measurement. Detailed instructions are available in the kit insert, which should be read thoroughly before conducting the assay. The kit includes an Acetylcholinesterase Standard to establish a precise standard curve.

Protocol Summary:

  • Add standards or diluted samples to the black microtiter plate.
  • Introduce Assay Buffer and Reaction Mix to each well, ensuring complete mixing.
  • Incubate the plate at room temperature for 20 minutes, allowing the ThioStar® reagent to bind to the thiol generated by the interaction between the AChE substrate and the AChE in the samples.
  • After incubation, use a plate reader to detect the fluorescent signal at 510nm. Calculate AChE activity using the standard curve.


Acetylcholinesterase (AChE) is an enzyme critical for breaking down Acetylcholine (ACh). ACh is a key neurotransmitter in cholinergic neurons within the central and peripheral nervous systems. Enzyme AChE plays an important role in the development and function of the nervous system. It is intimately involved in regulating circadian rhythms and modulating memory processes.

Cholinergic neurotransmission, mediated by ACh, is particularly significant in conditions such as Alzheimer’s disease (AD), where impairment of this system is a notable feature. AChE inhibitors, which prevent the breakdown of ACh and thereby enhance cholinergic neuronal activity, are a primary treatment for AD. Beyond therapeutic applications, AChE inhibitors also enable some pesticides, underscoring the enzyme’s importance in both medical and agricultural contexts.

The DetectX® Acetylcholinesterase (AChE) Fluorescent Activity Kit is an essential tool for researchers studying neurobiology, pharmacology, and toxicology. It enables precise quantification of AChE activity, providing insights into cholinergic function and the mechanisms of diseases and treatments involving AChE.

Acetylcholinesterase (AChE) Fluorescent Activity Kit


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The DetectX® Acetylcholinesterase (AChE) Activity Kit is designed to quantitatively measure AChE activity in a variety of samples, utilizing a proprietary non-fluorescent molecule, ThioStar®.

SKU: K015-F1 Category: