• Catalog Number K072-H
  • Assay Type Sandwich ELISA
  • Sample Types Plasma
  • Sensitivity 12.71 pg/mL
  • Species Human
  • Assay Duration 2 Hours
  • Samples/Plate 40 in Duplicate
  • Readout Colorimetric, 450 nm


Assay Principle: 

The Adrenocorticotropic Hormone (ACTH) ELISA Kit quantitatively measures ACTH levels in plasma. This sandwich ELISA has a run time of 2 hours. Please read the complete kit insert for more information before performing this assay. Use our provided human ACTH standard to generate a standard curve for the assay.

Protocol Summary:

  • Add standards or diluted samples into the included transparent microtiter plate, coated with mouse anti-human ACTH.
  • Incubate the plate, covered and shaking, at room temperature for 1 hour.
  • After washing the plate, introduce the peroxidase-conjugated mouse anti-human ACTH monoclonal antibody.
  • Incubate again, covered and shaking, for 30 minutes at room temperature. This step facilitates the immunological reaction.
  • Post-incubation, wash the plate to remove excess conjugate and add the TMB substrate, which reacts with the bound conjugate to generate a detectable signal.
  • Determine ACTH concentrations by measuring the absorbance at 450nm using a plate reader and referencing the standard curve.


Adrenocorticotropic hormone (ACTH), also known as adrenocorticotropin or corticotropin, is a hormone in the hypothalamic-pituitary-adrenal (HPA) axis, produced in response to stress. It primarily influences the production and release of glucocorticoids (GCs). Stress-induced secretion of Corticotropin Releasing Hormone (CRH) stimulates pituitary ACTH secretion, which in turn activates adrenal zona cells to synthesize and release adrenal steroids, including aldosterone, GCs, and adrenal androgens. ACTH is the key regulator of cortisol and corticosterone, and its synthesis is influenced by the circadian rhythm.

Measuring plasma ACTH levels enables the differential diagnosis of several endocrine disorders, including pituitary Cushing’s disease, Addison’s disease, adrenal tumors, adrenal hyperplasia, and ectopic ACTH syndrome. The DetectX® Adrenocorticotropic Hormone (ACTH) ELISA Kit offers clinical researchers a reliable and sensitive tool for quantifying ACTH levels, aiding in the understanding and treatment of various HPA axis-related conditions.

Adrenocorticotropic Hormone (ACTH) ELISA Kits


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The DetectX® Adrenocorticotropic hormone (ACTH) ELISA Kits quantitatively measure ACTH present in plasma samples.

SKU: K072-H Category:

Research Areas