Aldosterone ELISA Kits

Research Areas

Documentation

Specifications

• Assay Type Competitive ELISA
• Sample Types Extracted Serum, Extracted Plasma (EDTA and Heparin), Urine, Saliva, Fecal Extracts, Tissue Culture Media
• Sensitivity 4.97 pg/mL
• Species Aldosterone is identical across species
• Assay Duration 2.5 Hours or Overnight
• Samples/Plate 40 in Duplicate
• Readout Colorimetric, 450 nm
• Standard Curve
• Description

  Assay Principle: 

  The Aldosterone ELISA Kit quantitatively measures aldosterone in extracted serum, extracted plasma (EDTA and Heparin), urine, saliva, fecal extracts, and tissue culture media. The Aldosterone ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

  Use our provided aldosterone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our donkey anti-sheep IgG antibody. Add the aldosterone peroxidase conjugate and the aldosterone polyclonal sheep antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-aldosterone antibody, the aldosterone antigen in the sample or standard, and the aldosterone-peroxidase conjugate. As the aldosterone concentration in the sample increases, the bound aldosterone-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

  After the 2-hour incubation, wash away the excess aldosterone-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound aldosterone-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the aldosterone concentration in the samples.

  Background:

  Aldosterone is a mineralocorticoid first isolated by Simpson and Tait’s husband and wife team at University College, London. Aldosterone controls the sodium-potassium balance through unidirectional salt reabsorption in various tissues and glands. It is synthesized from cholesterol in the zona glomerulosa of the adrenal cortex, and its secretion is regulated through the renin-angiotensin system. Angiotensin II and potassium stimulate primary secretion by increasing the steroid production rate. Peripheral aldosterone levels are dependent on age and body position. Aldosterone measurement is useful in investigating primary and secondary aldosteronism, including vascular disease, salt depletion, potassium loading, cardiac failure with ascites, and pregnancy.