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Specifications

  • Assay Type Activity Assay
  • Sample Types Serum, Non-EDTA Plasma
  • Sensitivity 0.063 mU/mL
  • Species Species Independent
  • Assay Duration 30 Minutes
  • Samples/Plate 40 samples in duplicate
  • Readout Colorimetric, 405 nm
  • Standard Curve
  • Description

    Assay Principle: 

    The Alkaline Phosphatase (ALP) Colorimetric Activity Kit quantitatively measures ALP activity in serum and plasma samples. The Alkaline Phosphatase (ALP) Colorimetric Activity Kit is an Activity Assay with a run time of 30 minutes. Please read the complete kit insert for more information before performing this assay.

    Use our provided Alkaline Phosphatase Standard to generate a standard curve for the assay. Pipette the standards and samples into the transparent microtiter plate. Add the Assay Buffer and pNPP Substrate to each well, tapping the plate to ensure sufficient mixing of reagents. Then incubate the plate at 37°C for 30 minutes. The color-generating reaction occurs between the Substrate and ALP in the samples.

    After the 30-minute incubation, use a plate reader to detect and record the generated signal at 405nm. Use the intensity and the standard curve to calculate the ALP activity in the samples.

    Background:

    Alkaline Phosphatase (ALP) is an enzyme that circulates throughout the bloodstream, helping de-phosphorylate various proteins in the body. Found in many higher organisms, ALP plays an active role in regulating many biological processes, ranging from metabolism, signal transduction, molecule transportation, and the expression of genetic information. It is found in the liver, bile duct, bones, kidneys, intestines, and placenta. The measurement of ALP activity is used to aid in studying physiological conditions and disease states, mostly involving the skeletal system and liver and the structure-activity relationships in inhibitor research. Physicians often check ALP levels to assess liver and gallbladder function and even the health of the bones. High ALP levels can indicate problems with the liver or gallbladder, such as a blockage in the bile ducts or gallstones. Low ALP levels could indicate a protein deficiency or malnutrition.

    Assay Kit developed by 21 Grams Assays, Inc., www.21GramsAssays.com.