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Specifications
- Assay Type Competitive ELISA
- Sample Types Saliva, Urine, Extracted Serum, Extracted Plasma
- Sensitivity 2.3 pg/mL
- Species Androstenedione is identical across species
- Assay Duration 2.5 Hours
- Samples/Plate 40 in Duplicate
- Readout Colorimetric, 450 nm
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Standard Curve
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Description
Assay Principle:
The Androstenedione ELISA Kit quantitatively measures Androstenedione in saliva, urine, extracted serum, and extracted plasma. The Androstenedione ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided Androstenedione standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our donkey anti-sheep IgG antibody. Add the Androstenedione peroxidase conjugate and the cGMP rabbit polyclonal antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-Androstenedione antibody, the Androstenedione antigen in the sample or standard, and the Androstenedione conjugate. As the Androstenedione concentration in the sample increases, the bound Androstenedione-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 2-hour incubation, wash away the excess Androstenedione-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound Androstenedione-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the Androstenedione concentration in the samples.
Background:
Androstenedione is a steroid hormone also known as androst-4-ene-3,17-dione. It is synthesized from dehydroepiandrosterone (DHEA) or 17-hydroxyprogesterone. By itself, Androstenedione is a weak androgen though it is a precursor for the biosynthesis of more potent androgens, such as testosterone and estrogens. Therefore, Androstenedione is a controlled drug and a supplement to enhance athletic performance and body energy.
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Structure
