• Catalog Number K070-H
  • Assay Type Competitive ELISA
  • Sample Types Saliva, Urine, Extracted Serum, Extracted Plasma
  • Sensitivity 2.3 pg/mL
  • Species Androstenedione is identical across species
  • Assay Duration 2.5 Hours
  • Samples/Plate 40 in Duplicate
  • Readout Colorimetric, 450 nm


Assay Principle: 

The DetectX® Androstenedione ELISA Kit quantitatively measures Androstenedione in saliva, urine, extracted serum, and extracted plasma. This competitive ELISA has a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay. Use our provided Androstenedione standard to generate a standard curve for the assay.

Protocol Summary:

  • Pipette standards or diluted samples into the provided transparent microtiter plate, coated with donkey anti-sheep IgG antibody.
  • Add Androstenedione peroxidase conjugate and cGMP rabbit polyclonal antibody.
  • Incubate the mixture, covered, at room temperature with shaking for 2 hours, allowing the immunological reaction between the Androstenedione antigen and the conjugate.
  • Post-incubation, wash the plate to remove excess conjugate and introduce the TMB substrate. The substrate reacts with the bound conjugate to produce a measurable signal.
  • Detect the signal at 450nm using a plate reader and calculate Androstenedione concentration based on the standard curve.


Androstenedione, chemically known as androst-4-ene-3,17-dione, is an important steroid hormone derived from dehydroepiandrosterone (DHEA) or 17-hydroxyprogesterone. While Androstenedione itself is a weak androgen, it is a primary precursor for the biosynthesis of more potent androgens like testosterone and many types of estrogen. This conversion process is integral to the endocrine system’s function and has several implications for both male and female physiology.

In men, Androstenedione is produced in the testes and adrenal glands, contributing to the development and maintenance of secondary sexual characteristics. In women, it plays a role in the ovarian production of estrogens, which are critical to reproductive health and the menstrual cycle. The balance of Androstenedione and its derivatives enables normal sexual and reproductive function.

Furthermore, artificial manipulation of Androstenedione levels play a role in enhancing athletic performance and increasing energy.  However, Androstenedione use as a performance-enhancing supplement is controversial and regulated, as elevated levels can have significant hormonal effects.

In clinical diagnostics, abnormal levels of Androstenedione can indicate adrenal tumors, polycystic ovary syndrome (PCOS), and congenital adrenal hyperplasia. Monitoring Androstenedione levels is therefore essential in the diagnostic evaluation of these disorders.

The DetectX® Androstenedione ELISA Kit offers precise and reliable quantification of Androstenedione levels in various sample types. This kit is a valuable tool for researchers in endocrinology, reproductive medicine, and sports science.

Androstenedione ELISA Kits

In Stock
1 Plate K070-H1
5 Plates K070-H5

Research Areas