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- Assay Type Detection Kit
- Sample Types Serum, Plasma, Urine, Cell Lysates, Tissue Homogenates
- Sensitivity 1.68 µg/mL
- Species All Species
- Assay Duration 2 Hours
- Samples/Plate 41 in Duplicate
- Readout Colorimetric, 560 nm
- Standard Curve
Protein determination is one of the most common operations performed in biochemical research. The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry assay, and relies on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to protein present. It has been shown that cysteine, cystine, tryptophan, tyrosine, and peptide bonds are able to reduce Cu2+ to Cu1+. BCA forms a purple-blue complex with Cu1+ in alkaline environments, thus providing a basis to monitor the reduction of alkaline Cu2+ by proteins.
The kit provides everything needed to measure protein content of a sample and the assay measures all types of proteins from all species. A bovine serum albumin (BSA) standard is provided to generate a standard curve for the assay and all samples should be read off of the standard curve. Samples are diluted in water and added to the wells. The BCA Color Solution is made by mixing the BCA Reagent with the BCA Enhancer. The BCA Color Solution is added to all wells and the plate incubated at 37°C. Protein in the samples reacts with the BCA Color Reagent to generate a purple colored product which is read at 560 nm.