BCA Protein Dual Range Colorimetric Detection Kit

Research Areas

Documentation

Specifications

• Assay Type Detection Kit
• Sample Types Serum, Plasma, Urine, Cell Lysates, Tissue Homogenates
• Sensitivity 1.68 µg/mL
• Species All Species
• Assay Duration 2 Hours
• Samples/Plate 41 in Duplicate
• Readout Colorimetric, 560 nm
• Standard Curve
• Description

  Assay Principle: 

  The BCA Protein Dual Range Colorimetric Detection Kit quantitatively measures total protein levels in serum, plasma, urine, cell lysates, and tissue homogenates. The BCA Protein Dual Range Colorimetric Detection Kit is a Detection Kit with a run time of 2 hours. Please read the complete kit insert for more information before performing this assay.

  Use our provided Bovine Serum Albumin Standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate. Add BCA Color Solution to each well, tapping the plate to ensure sufficient mixing of reagents. Then, incubate the mixture at 37°C for 2 hours. The color-generating reaction occurs between the BCA Color Reagent and Protein within the sample or standard.

  After the 2-hour incubation, use a plate reader to detect and record the generated signal at 560nm. Use the intensity and the standard curve to calculate the Protein concentration in the samples.

  Background:

  Protein determination is one of the most common operations performed in biochemical research. The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry assay. It relies on forming a Cu2+-protein complex under alkaline conditions, followed by reducing the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. Cysteine, cystine, tryptophan, tyrosine, and peptide bonds can reduce Cu2+ to Cu1+. BCA forms a purple-blue complex with Cu1+ in alkaline environments, thus providing a basis to monitor the reduction of alkaline Cu2+ by proteins.