Cortisone Enzyme Immunoassay Kit

The DetectX® Cortisone Enzyme Immunoassay kit is designed to quantitatively measure cortisone present in extracted dried fecal samples, urine, saliva, plasma, and serum samples. This kit measures total cortisone in serum and plasma and in extracted fecal samples. A cortisone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. A cortisone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to cortisone to each well. After a two hour incubation the plate is washed and TMB substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce a colored product. After a short incubation, the reaction is stopped and the intensity of the generated color is  measured at 450nm.