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- Assay Type Competitive ELISA
- Sample Types Serum, Plasma, Urine, Saliva, Fecal Extracts
- Sensitivity 29.0 pg/mL
- Species Cortisone is identical across species
- Assay Duration 2.5 Hours
- Samples/Plate 40 in Duplicate
- Readout Colorimetric, 450 nm
- Standard Curve
The Cortisone ELISA Kit quantitatively measures Cortisone in serum, plasma, urine, saliva, and fecal extracts. The Cortisone ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided cortisone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the cortisone peroxidase conjugate and the cortisone polyclonal rabbit antibody. Then incubate the mixture covered for 2 hours, shaking at room temperature. The immunological reaction occurs between the anti-cortisone polyclonal antibody, the cortisone antigen in the sample or standard, and the cortisone-peroxidase conjugate. As the cortisone concentration in the sample increases, the bound cortisone-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 2-hour incubation, wash away the excess cortisone-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound cortisone-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the cortisone concentration in the samples.
Cortisone (C21H28O5, Kendall’s Compound ‘E’) was by bovine suprarenal gland tissue extraction. Cortisol and cortisone concentrations vary due to the activity of two 11β-hydroxysteroid dehydrogenases (11β-HSD). 11β-HSD1 is found primarily in the liver, which converts Cortisone to cortisol, while 11β-HSD2 is found in tissues such as the kidney where cortisol receptor binding is required. This glucocorticoid “shuttle” helps to initiate and regulate the anti-inflammatory response.