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- Sample Types Cell and Tissue Lysates, Urine, Plasma, Saliva, Tissue Culture Media
- Species Cyclic GMP is identical across species
- Sensitivity 0.091 pmol/mL, 4.55 fmol/well
- Time to Answer 2.5 Hours
- Samples/Kit 38 or 230 in Duplicate
- Stability Liquid 4°C Stable Reagents
- Readout Colorimetric, 450 nm
- Standard Curve
Guanosine 3’, 5’-cyclic monophosphate (cyclic GMP; cGMP) is a critical and multifunctional second messenger present at levels typically 10-100 fold lower than cAMP in most tissues. Intracellular cGMP is formed by the action of the enzyme guanylate cyclase (GC) on GTP and degraded through phosphodiesterase hydrolysis. Guanylate cyclases are either soluble or membrane bound. Soluble GCs are nitric oxide responsive, whereas membrane bound GCs respond to hormones such as acetylcholine, insulin and oxytocin. Other chemicals like serotonin and histamine also cause an increase in cGMP levels. Cyclic GMP regulates cellular composition through cGMP-dependent kinase, cGMP-dependent ion channels or transporters, and through its hydrolytic degradation by phosphodiesterase.