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- Assay Type Competitive ELISA
- Sample Types Serum, Plasma, Urine, Saliva, Fecal Extracts, Tissue Culture Media
- Sensitivity 90.9 pg/mL
- Species DHEA-S is identical across species
- Assay Duration 2.5 Hours
- Samples/Plate 41 in Duplicate
- Readout Colorimetric, 450 nm
- Standard Curve
The DHEA-S ELISA Kit quantitatively measures DHEA-S in serum, plasma, urine, saliva, fecal extracts, and tissue culture media. The DHEA-S ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided DHEA-S standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our donkey anti-sheep IgG antibody. Add the DHEA-S peroxidase conjugate and the DHEA-S polyclonal sheep antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-DHEA-S antibody, the DHEA-S antigen in the sample or standard, and the DHEA-S-peroxidase conjugate. As the DHEA-S concentration in the sample increases, the bound DHEA-S-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 2-hour incubation, wash away the excess DHEA-S-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound DHEA-S-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the DHEA-S concentration in the samples.
Dehydroepiandrosterone sulfate (DHEA-S) is the primary C19 steroid secreted by the adrenal cortex and is a precursor to testosterone and estrogen biosynthesis. It is produced by adding a sulfate group to dehydroepiandrosterone (DHEA) catalyzed by the sulfotransferase enzymes SULT1A1 and SULT1E1, which produce estrone sulfate from estrone. Due to the presence of a 17-ketone group rather than a hydroxyl group, DHEA-S has relatively low androgenic activity. The bioactivity of DHEA-S may be high due to its serum concentrations being 100-1,000-fold higher than testosterone or DHEA, in addition to its weak affinity for sex-hormone binding globulin.