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Specifications
- Assay Type Competitive ELISA
- Sample Types Saliva, Urine, Extracted Serum, Extracted Plasma, Fecal Extracts, Tissue Culture Media
- Sensitivity 0.120 ng/mL
- Species Epiandrosterone is identical across species
- Assay Duration 2.5 Hours
- Samples/Plate 40 in Duplicate
- Readout Colorimetric, 450 nm
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Standard Curve
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Description
Assay Principle:
The Epiandrosterone ELISA Kit quantitatively measures Epiandrosterone in saliva, urine, extracted serum, extracted plasma, fecal extracts, and tissue culture media. The Epiandrosterone ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided Epiandrosterone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the Epiandrosterone peroxidase conjugate and the Epiandrosterone rabbit polyclonal antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-Epiandrosterone antibody, the Epiandrosterone antigen in the sample or standard, and the Epiandrosterone conjugate. As the Epiandrosterone concentration in the sample increases, the bound Epiandrosterone-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 2-hour incubation, wash away the excess Epiandrosterone-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound Epiandrosterone-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the Epiandrosterone concentration in the samples.
Background:
Epiandrosterone is a naturally occurring metabolite of dehydroepiandrosterone (DHEA) found in most mammals and produced via the action of the 5α-reductase enzyme. It is a weak androgen formed primarily in peripheral tissues, released into circulation, and ultimately excreted in the urine. Epiandrosterone can inhibit glucose-6-phosphate dehydrogenase (G6PD) and lower intracellular NADPH levels. Its inhibition of G6PD correlates to alterations in heart contractibility. It also attenuates NO-evoked relaxation of the pulmonary artery. Researchers have begun to evaluate Epiandrosterone function in cell metabolism, cardiac functions, and prostate cancer research. Evaluating Epiandrosterone concentrations helps investigators monitor gonadal activity and sexual behavior in males.
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Structure
