- Multi Species
- USE - Measure Epiandrosterone in a Variety of Matrices
- SAMPLE - Saliva, Urine, Extracted Serum or Plasma, Dried Fecal Extracts, TCM
- SAMPLES/KIT - 40 or 232 in Duplicate
- SENSITIVITY - 0.120 ng/mL
The DetectX® Epiandrosterone Immunoassay kit uses a specifically generated antibody to measure Epiandrosterone in urine, saliva, and fecal samples, or in extracted serum and plasma. This kit is not recommended for serum or plasma samples without extraction. The kit will also quantitatively measure epiandrosterone present in reconstituted buffer samples and tissue culture media samples. Please read the complete kit insert before performing this assay. An epiandrosterone standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An Epiandrosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to epiandrosterone to each well. After a two hour incubation the plate is washed and substrate added. The substrate reacts with the bound epiandrosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the epiandrosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.