Specifications

  • Catalog Number KB30-H
  • Assay Type Competitive ELISA
  • Sample Types Serum, Plasma
  • Sensitivity 2.21 pg/mL
  • Species Estradiol is identical across species
  • Assay Duration 2.5 Hours
  • Samples/Plate 40 in Duplicate
  • Readout Colorimetric, 450 nm

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Assay Principle: 

The Serum 17β-Estradiol ELISA Kit quantitatively measures free 17β-Estradiol in plasma and serum. This competitive ELISA has a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay. Use our provided 17β-Estradiol standard to generate a standard curve for the assay.

Protocol Summary:

  • Add standards or diluted samples to the transparent microtiter plate coated with donkey anti-sheep IgG antibody.
  • Introduce the 17β-Estradiol peroxidase conjugate and 17β-Estradiol sheep polyclonal antibody into each well.
  • Incubate the plate covered and shaking at room temperature for 1 hour, facilitating the immunological reaction.
  • Post-incubation, wash the plate to remove excess conjugate.
  • Add the TMB substrate, which reacts with the bound conjugate to produce a measurable signal.
  • Detect the signal at 450nm using a plate reader and determine the 17β-Estradiol concentration based on the standard curve.

Background:

Estradiol (E2, 17β-estradiol), the primary estrogen in humans, plays an important role in both female and male physiology. In females, estradiol is vital for the development and maintenance of reproductive tissues, menstrual cycle regulation, and overall fertility. It also contributes to bone strength, cardiovascular health, and skin integrity. In males, estradiol, derived from the metabolic conversion of testosterone, regulates libido, erectile function, and spermatogenesis.

The measurement of estradiol levels in plasma and serum is critical in clinical diagnostics and research. In women, serum estradiol measurements reflect ovarian activity, helping diagnose conditions like amenorrhea, menstrual dysfunction, hypoestrogenism, and menopause. These measurements also inform fertility treatments to monitor follicular development and assess the efficacy of ovarian stimulation protocols. For men, assessing estradiol levels can help in diagnosing hypogonadism, gynecomastia, and other hormonal imbalances.

Furthermore, abnormal estradiol levels can be indicative of various pathological conditions. Elevated estradiol levels may suggest estrogen-producing tumors, while low levels could be associated with osteoporosis or cardiovascular risks. In both genders, estradiol measurements are crucial for evaluating hormonal therapies’ effectiveness and guiding clinical decisions.

The DetectX® Serum 17β-Estradiol ELISA Kit offers a sensitive, specific, and rapid method for quantifying estradiol levels in serum and plasma samples. This makes it a valuable tool for endocrinologists, reproductive health specialists, and researchers studying hormone-related diseases and therapies.

Serum 17β-Estradiol ELISA Kits

$386.00$1,544.00

In Stock

The DetectX® Serum 17β-Estradiol ELISA Kits quantitatively measure free 17β-Estradiol in serum and plasma.

SKU: KB30-H Category:

Research Areas