- Multi Species
- Use - Measure Free 17β-Estradiol in Serum and Plasma
- Sample - Serum and Plasma - Without Extraction
- Samples/Kit - 40 or 232 in Duplicate
- Selective - Low Cross Reactivity to Other Steroids
The DetectX® Serum Estradiol ELISA Kit is designed to quantitatively measure Free 17β-estradiol present in serum and plasma samples. An estradiol standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. An estradiol-peroxidase conjugate is added to the wells. The binding reaction for the EIA/ELISA is initiated by the addition of a sheep antibody to estradiol to each well. After incubation the plate is washed and substrate is added. The substrate reacts with the bound estradiol-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color from the EIA/ELISA is detected in a microtiter plate reader capable of measuring 450nm wavelength.
17β-Estradiol (E2) is a key regulator of growth, differentiation, and function in a wide array of tissues, including the male and female reproductive tracts, mammary gland, brain, skeletal and cardiovascular systems. The predominant biological effects of E2 are mediated through two distinct intracellular receptors, ERa and ERß, each encoded by unique genes possessing the functional domain characteristics of the steroid/thyroid hormone superfamily of nuclear receptors. ERa is the predominant form expressed in the breast, uterus, cervix, and vagina. ERß exhibits a more limited pattern and is primarily expressed in the ovary, prostate, testis, spleen, lung, hypothalamus, and thymus. Estradiol also influences bone growth, brain development and maturation, and food intake, and it is also critical in maintaining organ functions during severe trauma. In plasma, estradiol is bound to serum proteins such as albumin and sex hormone-binding globulin. Just over 2% of E2 is free and biologically active, the percentage remaining constant throughout the menstrual cycle. Estradiol is conjugated in the liver to sulfate and glucuronide derivatives and excreted. Deactivation includes conversion to less-active estrogens, such as estrone and estriol. Estriol is the major urinary metabolite.