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- Assay Type Competitive ELISA
- Sample Types Serum, Plasma
- Sensitivity 2.21 pg/mL
- Species Estradiol is identical across species
- Assay Duration 2.5 Hours
- Samples/Plate 40 in Duplicate
- Readout Colorimetric, 450 nm
- Standard Curve
The Serum 17β-Estradiol ELISA Kit quantitatively measures free 17β-Estradiol in plasma and serum. The Serum 17β-Estradiol ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided 17β-Estradiol standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our donkey anti-sheep IgG antibody. Add the 17β-Estradiol peroxidase conjugate and the 17β-Estradiol sheep polyclonal antibody. Then incubate the mixture covered at room temperature, shaking for 1 hour. The immunological reaction occurs between the anti-17β-Estradiol antibody, the 17β-Estradiol antigen in the sample or standard, and the 17β-Estradiol conjugate. As the 17β-Estradiol concentration in the sample increases, the bound 17β-Estradiol-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 1-hour incubation, wash away the excess 17β-Estradiol-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound 17β-Estradiol-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the 17β-Estradiol concentration in the samples.
Estradiol (E2, 17β-estradiol, or oestradiol) is the predominant sex hormone present in females. It is also present in males, being produced as an active metabolic product of testosterone. It represents the primary estrogen in humans. Estradiol has not only a critical impact on reproductive and sexual functioning but also affects other organs. Serum estradiol measurements in women reflect the activity of the ovaries primarily. As such, they help detect baseline estrogen in women with amenorrhea or menstrual dysfunction and detect the state of hypoestrogenism and menopause. Furthermore, estrogen monitoring during fertility therapy assesses follicular growth. Estrogen-producing tumors and precocious puberty samples will demonstrate persistent high levels of estradiol and other estrogens.