Assay Principle:

The DetectX® Estrone ELISA Kit quantitatively measures estrone in fecal extracts, urine, and tissue culture media. This competitive ELISA has a run time of 2.5 hours, providing a reliable and efficient approach for estrone assessment. Read the complete kit insert thoroughly before running the assay. The kit includes an Estrone standard to establish an accurate standard curve.

Protocol Summary:

• Introduce standards or diluted samples into the provided transparent microtiter plate coated with goat anti-rabbit IgG antibody.
• Add Estrone peroxidase conjugate and Estrone polyclonal rabbit antibody to initiate the immunological reaction.
• Incubate the mixture covered at room temperature, with shaking, for 2 hours. The reaction varies inversely with estrone concentration in the sample.
• After incubation, wash away excess conjugate and add the TMB substrate. The substrate reacts with the bound conjugate to produce a detectable signal.
• Use a plate reader to detect the signal at 450nm. Calculate the estrone concentration based on the standard curve.

Background:

Estrone (C18H22O2) is a C-18 steroid hormone and one of the three naturally occurring estrogens, alongside estradiol and estriol. It is primarily produced from androstenedione originating from the gonads or adrenal cortex, and also from estradiol by 17-hydroxysteroid dehydrogenase. Additionally, the aromatase (CYP19) enzyme converts androstenedione into estrone.

Estrone plays a significant role in breast cancer tissue, expressed in both the stromal and carcinoma or parenchymal components. In premenopausal mammals, estrone concentrations fluctuate with the menstrual cycle. The ovaries supply over 50% of estrone in premenopausal women.

In men, non-supplemented postmenopausal women, and preadolescent children, a substantial portion of estrone is derived from the peripheral tissue conversion of androstenedione. The interconversion of estrone and estradiol also occurs in peripheral tissues, making its measurement crucial in understanding various physiological processes and conditions.

The DetectX® Estrone ELISA Kit is a valuable tool for researchers and clinicians in endocrinology, reproductive health, and oncology.