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Specifications
- Assay Type Competitive ELISA
- Sample Types Fecal Extracts, Urine, Tissue Culture Media
- Sensitivity 22.4 pg/mL
- Species Estrone is identical across species
- Assay Duration 2.5 Hours
- Samples/Plate 39 in Duplicate
- Readout Colorimetric, 450 nm
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Standard Curve
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Description
Assay Principle:
The Estrone ELISA Kit quantitatively measures Estrone in fecal extracts, urine, and tissue culture media. The Estrone ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided Estrone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the Estrone peroxidase conjugate and the Estrone polyclonal rabbit antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-Estrone antibody, the Estrone antigen in the sample or standard, and the Estrone-peroxidase conjugate. As the Estrone concentration in the sample increases, the bound Estrone-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 2-hour incubation, wash away the excess Estrone-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound Estrone-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the Estrone concentration in the samples.
Background:
Estrone, C18H22O2, is a C-18 steroid hormone and is one of the three naturally occurring estrogens; the others being estradiol and estriol. Estrone is produced primarily from androstenedione from the gonads or the adrenal cortex and estradiol by 17-hydroxysteroid dehydrogenase. Androstenedione is also converted into Estrone by aromatase (CYP19) to Estrone. The stromal and carcinoma or parenchymal components of breast cancer tissue express Estrone. Estrone concentrations in premenopausal mammals fluctuate according to the menstrual cycle. In premenopausal women, the ovaries supply more than 50% of the Estrone. In prepubertal children, men, and non-supplemented postmenopausal women, a significant portion of Estrone is derived from peripheral tissue conversion of androstenedione. Interconversion of Estrone and estradiol also occurs in peripheral tissue.
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Structure
