AbX™ DyLight® 549-labeled Glutathione (GSH) Mouse Monoclonal Antibody is produced from our Protein A purified antibody by reaction with DyLight® 549 NHS ester. It will fluorescently label GSH-protein complexes under non-reducing conditions. DyLight® 549 is similar to Alexa 555, Cy3, or TRITC.

Glutathione (GSH) is the highest concentration non-protein thiol in mammalian cells and is present in concentrations of 0.5 to 10 mM. GSH plays a key role in many biological processes, including the synthesis of proteins and DNA, the transport of amino acids, and the protection of cells against oxidation. Harmful hydrogen peroxide cellular levels are minimized by the enzyme glutathione peroxidase (GP) using GSH as a reductant. The oxidized GSH dimer, GSSG, is formed from GSH and peroxide by the GP reaction. An important role of GSSG in the NFkB activating signal cascade is suggested by the fact that the potent NFkB inducer TPA increases intracellular GSSG levels and GSSG/GSH ratios.