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Specifications
- Assay Type Detection Kit
- Sample Types Urine, Water, Tissue Culture Media
- Sensitivity 0.715 μM
- Species Formaldehylde is identical across species
- Assay Duration 30 Minutes
- Samples/Plate 40 in Duplicate
- Readout Fluorescent, 510 nm emission / 450 nm excitation
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Standard Curve
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Description
Assay Principle:
The Formaldehyde Fluorescent Detection Kit quantitatively measures Formaldehyde levels in urine, water, and tissue culture media. The Formaldehyde Fluorescent Detection Kit is a Detection Kit with a run time of 30 minutes. Please read the complete kit insert for more information before performing this assay.
Use our provided Formaldehyde standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a black microtiter plate. Add DetectX® Formaldehyde Reagent to each well tapping the plate to ensure sufficient mixing of reagents. Then incubate the mixture covered at 37°C for 30 minutes. The fluorescent reaction occurs between the DetectX® Formaldehyde Reagent and the Formaldehyde within the sample or standard.
After the 30-minute incubation, use a plate reader to detect and record the generated fluorescent signal. Use the intensity and the standard curve to calculate the Formaldehyde concentration in the samples.
Background:
Formaldehyde (methanal), H2C=O, is a colorless, flammable, strong-smelling gas. Formaldehyde is commonly used as an industrial fungicide, germicide, disinfectant, and preservative in mortuaries and medical laboratories. It is an important industrial chemical used to manufacture building materials and to produce many household products. The US generates approximately 3 x 109 Kg of Formaldehyde annually.
Materials containing Formaldehyde can release formaldehyde gas or vapor into the air. Burning wood, kerosene, natural gas, cigarettes, automobile emissions, and natural processes also emits Formaldehyde. Occupational exposure to Formaldehyde by inhalation is mainly from three sources: thermal or chemical decomposition of formaldehyde-based resins, formaldehyde emission from aqueous solutions (for example, embalming fluids), and the production of Formaldehyde resulting from combustion. Formaldehyde can be toxic, allergenic, and carcinogenic. Because many construction materials contain formaldehyde resins, it is a more common indoor air pollutant.
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