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- Assay Type Detection Kit
- Sample Types Buffers
- Sensitivity 4.62 nM
- Assay Duration 30 Minutes
- Samples/Plate 39 in Duplicate
- Readout Fluorescent, 510 nm emission / 370-410 nm excitation
- Standard Curve
Assay Principle: The DetectX® Thiol Detection Kit is designed to quantitatively measure thiol groups generated or present in biological samples. Please read the complete kit insert before performing the assay.
Background: Free thiols in biological systems have important roles. Oxidatively-modified thiol groups of cysteine residues are known to modulate the activity of a growing number of proteins. As such, it is important to be able to accurately determine the extent of modification of specific amino acids, such as cysteine residues. This is especially difficult in a complex protein sample, especially in the presence of chaotropic agents such as guanidine hydrochloride. Typical methods using Ellman’s reagent do not have sufficient sensitivity to allow economical detection of free SH groups.