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- Assay Type Detection Kit
- Sample Types API scavenging steps
- Assay Duration 30 Minutes
- Samples/Plate 41 in Duplicate
- Readout Fluorescent, 520 nm Emission / 485 nm Excitation
- Standard Curve
The Palladium API Screening Fluorescent Detection Kit quantitatively measures Palladium throughout API scavenging steps. The Palladium API Screening Fluorescent Detection Kit is a Detection Kit with a run time of 30 minutes. Please read the complete kit insert for more information before performing this assay.
Use our provided Palladium standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a black microtiter plate. Add PdX™ Palladium Detection Reagent and the Sodium Borohydride Reagent solution to each well, tapping the plate to ensure sufficient mixing of reagents. Then incubate the mixture covered at room temperature for 30 minutes. The fluorescent reaction occurs between the PdX™ Palladium Detection Reagent and the reducing Pd within the sample or standard.
After the 30-minute incubation, use a plate reader to detect and record the generated fluorescent signal. Use the intensity and the standard curve to calculate the Palladium concentration in the samples.
Many pharmaceutical processes use palladium (Pd) compounds for synthetic transformations. Palladium (Pd) compounds are great catalysis of carbon-carbon and carbon-heteroatom coupling reactions. These reactions are popular for pharmaceutical processes, utilizing various functional groups to build complicated molecules. However, palladium-catalyzed reactions are problematic as the palladium can persist in the isolated active pharmaceutical ingredient (API) product. Current FDA and EMEA regulations limit platinum group (Pt, Pd, Ir, Rh, Ru, Os) metal contamination, as a whole, to less than 5 ppm. Standard methods of Pd API evaluation require expensive instrumentation and highly trained scientists to operate. These methods also suffer from probe cross-contamination and require scrupulous clean-up methodologies.