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- Sample Types Serum, Plasma (EDTA and Heparin), Cell Lysates, RBCs
- Time to Answer 20 minute End Point or Kinetic Assay
- Samples/Kit 41 in Duplicate
- Sensitivity 9 µU/mL
- Stability Liquid 4ºC stable reagents
- Readout Fluorescent, 510 nm emission / 370-410 nm excitation
- Standard Curve
The most widely used procedure to measure GR is to monitor the oxidation of NADPH as a decrease in absorbance at 340nm. However many biological molecules absorb light at 340 nm, plus the detection system gives very low OD readings. The Arbor Assays’ DetectX® Glutathione Reductase Fluorescent Activity kit overcomes these problems of measuring GR activity by the direct detection of the GSH formed from oxidized glutathione.
Glutathione reductase (GR) plays an indirect but essential role in the prevention of oxidative damage within the cell by helping to maintain appropriate levels of intracellular glutathione (GSH). GSH, in conjunction with the enzyme glutathione peroxidase (GP), is the acting reductant responsible for minimizing harmful hydrogen peroxide. The regeneration of GSH is catalyzed by GR. GR is a ubiquitous 100-120 kDa dimeric flavoprotein that catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione, using β-nicotinamide dinucleotide phosphate (NADPH) as the hydrogen donor. NADPH has been suggested to also act as an indirectly operating antioxidant, given its role in the recycling of GSSG to GSH and thus maintaining the antioxidative power of glutathione.