Specifications

  • Use Detection of HDM Activity; LSD1 and Jumonji type
  • Sample Types Lysates, Purified Enzyme Systems
  • Calibrated Measure demethylation product, Formaldehyde
  • Samples/Kit 89 in Duplicate
  • Stability Liquid 4˚C Stable Reagents
  • Readout Fluorescent, 510 nm emission / 450 nm excitation
  • Standard Curve
  • Description

    Histone demethylases (HDMs) catalyze the site-specific demethylation of methyl-lysine residues in histones to dynamically regulate chromatin structure, gene expression, and potentially other genomic functions. At present, there are two known classes of HDMs: the flavin adenine nucleotide (FAD)-dependent Lysine Specific Demethylase 1 (LSD1) family and the Fe(II)-dependent Jumonji C (JmjC) family. Although they employ different cofactors and catalytic mechanisms, both classes produce formaldehyde as a byproduct of the demethylation reaction. Despite their biological importance, HDMs have proven difficult to quantitatively assay owing to their relatively low turnover numbers, hindering our understanding of their kinetic properties, substrate specificities, and reaction mechanisms. 

     The DetectX® Universal Histone Demethylase (HDM) Fluorescent Activity Kit allows activity measurement of any Histone Demethylase system by measuring the formaldehyde generated by demethylation in low, medium and high throughput fashion. Run the demethylase reaction and after completion, simply add the supplied Formaldehyde Detection Reagent to each well and read the fluorescent signal generated. Lab-to-lab comparisons are possible with inclusion of a formaldehyde reference standard.