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- Assay Type Activity Assay
- Sample Types Any P450 system or biological system producing demethylation product, Formaldehyde
- Assay Duration 45 Minutes
- Samples/Plate 41 in Duplicate
- Readout Fluorescent, 510 nm Emission / 450 nm Excitation
- Standard Curve
Assay Principle: The P450 Fluorescent Activity kit is designed to quantitatively measure the enzymatic activity of formaldehyde-producing Cytochrome P450s. No separation or washing is required. The kit has been validated for several P450 systems. This kit should work with any biological system that produce formaldehyde as a product of demethylation. Please read the complete kit insert before performing this assay
Background: Cytochrome P450 enzymes, along with cofactors, function to metabolize drugs and play a crucial role in the development of new drug entities, as drug interactions commonly inhibit enzyme activities. The enzymes are a superfamily of heme containing enzymes. They display tremendous diversity regarding substrate specificity and catalytic activity by using both exogenous and endogenous compounds as substrates in their reactions and forming part of multicomponent electron transfer reactions.