Hemoglobin Colorimetric Detection Kit

Research Areas

Documentation

Specifications

• Assay Type Detection Kit
• Sample Types Whole Blood, RBCs
• Sensitivity 0.021 g/dL, 0.21 mg/mL
• Species Identical across all species
• Assay Duration 30 minutes
• Samples/Plate 40 in Duplicate
• Readout Colorimetric, 560-580 nm
• Standard Curve
• Description

  Assay Principle: 

  The Hemoglobin Colorimetric Detection Kit quantitatively measures all forms of hemoglobin levels in whole blood and RBCs. The Hemoglobin Colorimetric Detection Kit is a Detection Kit with a run time of 30 minutes. Please read the complete kit insert for more information before performing this assay.

  Use our provided Hemoglobin standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate. Add DetectX® Hemoglobin Detection Reagent to each well, tapping the plate to ensure sufficient mixing of reagents. Then incubate the mixture at room temperature for 30 minutes. The color-generating reaction occurs between the DetectX® Hemoglobin Detection Reagent and the Hemoglobin within the sample or standard. 

  After the 30-minute incubation, use a plate reader to detect and record the generated signal at 560-580nm. Use the intensity and the standard curve to calculate the Hemoglobin concentration in the samples.

  Background:

  Hemoglobin (Hgb) is a red erythrocyte protein complex responsible for transporting oxygen in the blood. Two sets of identical pairs of subunits comprise the Hemoglobins complex. Each of which binds to an iron-porphyrin group commonly called heme. Heme binds and releases oxygen or carbon dioxide in response to slight changes in local gas tension. Hemoglobin values are associated with conditions such as anemias (low Hgb), erythrocytosis (high Hgb), thalassemia (aberrant chain synthesis), and sickling disorders (abnormal complex shape).