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- Assay Type Detection Kit
- Sample Types Whole Blood, RBCs
- Sensitivity 0.021 g/dL, 0.21 mg/mL
- Species Human, Chicken, Dogfish
- Assay Duration 30 minutes
- Samples/Plate 40 in Duplicate
- Readout Colorimetric, 560-580 nm
- Standard Curve
All forms of hemoglobin are rapidly converted to a single stable form that is measured photometrically at 560-580 nm. A human hemoglobin standard is provided to generate a standard curve for the assay.
Hemoglobin (Hgb) is an erythrocyte protein complex comprised of two sets of identical pairs of subunits, each of which bind an iron-porphyrin group commonly called heme. Heme binds and releases oxygen or carbon dioxide in response to slight changes in local gas tension. Hemoglobin values are associated with a variety of conditions ranging from anemias (low Hgb), erythrocytosis (high Hgb), thalassemias (aberrant chain synthesis), and sickling disorders (abnormal complex shape).