Specifications

  • Assay Type Competitive ELISA
  • Sample Types Cell Lysates, Urine, Plasma, Saliva, Tissue, Tissue Culture Media
  • Sensitivity 0.119 pmol/mL (Regular), 0.015 pmol/mL (Acetylated)
  • Species Cyclic AMP is identical across species
  • Assay Duration 2 Hours
  • Samples/Plate 41 in Duplicate (Regular), 38 in Duplicate (Acetylated)
  • Readout Chemiluminescent
  • Standard Curve
  • Description

    Adenosine-3’, 5’-cyclic monophosphate, or cyclic AMP (cAMP), is one of the most important second messengers and a key intracellular regulator. It was discovered by Sutherland and Rall in 1957. Cyclic AMP functions as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH. Adenylate cyclase is activated by the hormones glucagon and adrenaline, and by G protein. Liver adenylate cyclase responds more strongly to glucagon and muscle adenylate cyclase responds more strongly to adrenaline. cAMP decomposition into AMP is catalyzed by the enzyme phosphodiesterase. The Human Metabolome Database lists 166 metabolic enzymes that convert cAMP.

    This kit is multi-format and can detect both acetylated and non-acetylated cAMP in a variety of sample types.