Specifications

  • Assay Type Competitive ELISA
  • Sample Types Serum, Plasma, Fecal Extracts, Urine, Tissue Culture Media
  • Sensitivity 20.8 pg/mL
  • Species PGFM is identical across species
  • Assay Duration 1.5 Hours
  • Samples/Plate 39 in Duplicate
  • Readout Colorimetric, 450 nm
  • Standard Curve
  • Description

    Uterine and placental Prostaglandin F2 (PGF2) is involved in the regulation of reproductive and pregnancy-related processes such as embryonic development, initiation of parturition, and resumption of ovarian activity. In domestic ruminants, uterine tissue is a primary source of PGF2 and secretion of uterine PGF2 is a key regulator for the cyclical regression of the corpus luteum. Prostaglandin F2 is metabolized to PGFM (13,14-dihydro-15-keto-PGF2) during passage through the lungs. PGFM has a longer half-life in peripheral circulation than PGF2 and has been applied as a useful analytical marker of PGF2. PGFM is a useful non-invasive marker of pregnancy when measured in both urine and fecal samples. It has been shown to be a precise, practical method for this application in these matrices. Fecal PGFM analyses may allow pregnancy diagnosis in captive and free-ranging felids as well as pandas and other species.