Progesterone ELISA Kit
Number of Plates
- Catalog Number K025-H
- Assay Type Competitive ELISA
- Sample Types Urine, Fecal Extracts, Tissue Culture Media
- Sensitivity 25.3 pg/mL
- Species Progesterone is identical across all species
- Assay Duration 2.5 Hours
- Samples/Plate 39 in Duplicate
- Readout Colorimetric, 450 nm
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Assay Principle:
The DetectX® Progesterone ELISA Kit provides an efficient method for quantitatively measuring progesterone in fecal extracts, urine, and tissue culture media. This is a competitive ELISA with a run time of 2.5 hours, Read the kit insert for detailed instructions before starting the assay. The kit includes a progesterone standard to create an accurate standard curve.
Protocol Summary:
- Add standards or diluted samples to the supplied microtiter plate coated with goat anti-mouse IgG antibody.
- Add progesterone peroxidase conjugate and progesterone monoclonal mouse antibody to initiate the reaction.
- Incubate the mixture at room temperature with shaking for 2 hours.
- After incubation, wash away excess conjugate and add the TMB substrate. TMB substrate reacts with the bound conjugate to produce a detectable signal.
- Use a plate reader to measure the signal at 450nm and calculate progesterone concentration using the standard curve.
Background:
Progesterone (C21H30O2), also known as P4 (pregn-4-ene-3,20-dione), is a C-21 steroid hormone that plays a central role in the regulation of reproduction in mammals and many other vertebrate species1. It is produced primarily by the corpus luteum and placenta and is involved in the regulation of the reproductive cycle, preparation of the uterus for embryo implantation, and maintenance of pregnancy.
In addition to its reproductive functions, Progesterone also influences physiological processes in several tissues, including the uterus, ovary, mammary gland, brain, cardiovascular system, and bone2. Because Progesterone concentrations vary predictably during reproductive cycles and pregnancy, measurement of Progesterone is widely used to monitor reproductive status, ovarian activity, and endocrine function across a variety of species. Progesterone and its metabolites can be measured in several biological matrices3, including urine, fecal extracts, and tissue culture media, enabling both invasive and non-invasive approaches to endocrine monitoring.
