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Specifications
- Assay Type Competitive ELISA
- Sample Types Serum, Plasma, Urine, Fecal Extracts, Tissue Culture Media
- Sensitivity 47.9 pg/mL
- Species Progesterone is identical across all species
- Assay Duration 2.5 Hours
- Samples/Plate 39 in Duplicate
- Readout Colorimetric, 450 nm
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Standard Curve
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Description
Assay Principle:
The Progesterone ELISA Kit quantitatively measures Progesterone in serum, plasma, fecal extracts, urine, and tissue culture media. The Progesterone ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided Progesterone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-mouse IgG antibody. Add the Progesterone peroxidase conjugate and the Progesterone monoclonal mouse antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-Progesterone monoclonal antibody, the Progesterone antigen in the sample or standard, and the Progesterone-peroxidase conjugate. As the Progesterone concentration in the sample increases, the bound Progesterone-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 2-hour incubation, wash away the excess Progesterone-peroxidase and add the TMB substrate. The TMB substrate reacts with the bound Progesterone-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the Progesterone concentration in the samples.
Background:
Progesterone, also known as P4 (Pregn-4-ene-3,20-dione), is a C21 steroid hormone involved in the female menstrual cycle, gestation, and embryogenesis of humans and other species. It belongs to a class of hormones called progestogens and is the major naturally occurring progestogen.
Progesterone is an essential regulator of human female reproductive function in the uterus, ovary, mammary gland, and brain. It also plays vital roles in non-reproductive tissues such as the cardiovascular, skeletal, and central nervous systems. Two isoforms of the nuclear progesterone receptor (PR), PR-A and PR-B, dictate progesterone action. Various healthy and cancerous breast tissue cells from humans, rats, and mice express PR-A and PR-B. Progesterone also has neurotrophic roles in the peripheral nervous system. It activates the growth and maturation of axons and stimulates the repair and replacement of myelin sheaths in regenerating nerve fibers.