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- Assay Type Competitive ELISA
- Sample Types Fecal Extracts, Urine, Tissue Culture Media
- Sensitivity 39.6 pg/mL
- Species Estradiol is identical across species
- Assay Duration 2.5 Hours
- Samples/Plate 41 in Duplicate
- Readout Colorimetric, 450 nm
- Standard Curve
The Estradiol ELISA Kit quantitatively measures Estradiol in urine, dried fecal extracts, and tissue culture media. The Estradiol ELISA Kit is a competitive ELISA with a run time of 2.5 hours. This kit provides a noninvasive Estrogen Assessment. Please read the complete kit insert for more information before performing this assay.
Use our provided Estradiol standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the Estradiol peroxidase conjugate and the Estradiol polyclonal rabbit antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-Estradiol polyclonal antibody, the Estradiol antigen in the sample or standard, and the Estradiol-peroxidase conjugate. As the Estradiol concentration in the sample increases, the bound Estradiol-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 2-hour incubation, wash away the excess Estradiol-peroxidase and add the TMB substrate. The TMB substrate reacts with the bound Estradiol-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the Estradiol concentration in the samples.
Estradiol (E2, 17β-estradiol, or oestradiol) is the predominant sex hormone present in females. It also acts as an active metabolic product of testosterone in males. Estradiol has not only a critical impact on reproductive and sexual functioning but also affects other organs. Serum estradiol measurements in women primarily reflect the activity of the ovaries. As such, they are helpful in the detection of baseline estrogen in women with amenorrhea or menstrual dysfunction and detect the state of hypoestrogenism and menopause. Furthermore, estrogen monitoring during fertility therapy assesses follicular growth. Estrogen-producing tumors and precocious puberty samples will demonstrate persistent high levels of Estradiol and other estrogens.