Specifications

  • Assay Type Activity Assay
  • Sample Types Serum, Urine
  • Sensitivity 3.26 mU/mL
  • Species Species Independent
  • Assay Duration 1 Hour
  • Samples/Plate 41 in Duplicate
  • Readout Colorimetric, 560 nm
  • Standard Curve Ceruloplasmin Colorimetric Activity Kit
  • Description

    Assay Principle: 

    The Ceruloplasmin Colorimetric Activity Kit quantitatively measures Ceruloplasmin activity in serum and plasma samples. The Ceruloplasmin Colorimetric Activity Kit is an Activity Assay with a run time of 60 minutes. Please read the complete kit insert for more information before performing this assay.

    Use our provided Ceruloplasmin Standard to generate a standard curve for the assay. Pipette the standards and samples into the transparent microtiter plate. Add the Assay Buffer and reconstituted Cp Substrate solution to each well, tapping the plate to ensure sufficient mixing of reagents. Then incubate the plate at 30°C for 60 minutes. The color-generating reaction occurs between the Ceruloplasmin Substrate and the Ceruloplasmin present in the samples.

    After the 60-minute incubation, use a plate reader to detect and record the generated signal at 560nm. Use the intensity and the standard curve to calculate the Ceruloplasmin activity in the samples.

    Background:

    Ceruloplasmin (Cp) is an acute phase multicopper oxidase enzyme that is protective in responses to immune-provoking stimuli and is also associated with reproduction. Estrogens alter the subcellular distribution of copper in the liver, increasing plasma copper levels and subsequent ceruloplasmin synthesis. Cp serum levels increase during normal pregnancy in some species, possibly as a protection against the oxidative costs of reproduction. In giant pandas and some felids, urinary Cp activity is elevated in pregnant vs. pseudopregnant animals beginning in the first week of gestation and continuing throughout the luteal phase.