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- Assay Type Competitive ELISA
- Sample Types Urine, Tissue Culture Media, Fecal Extracts
- Species Estrone-3-glucuronide (E1G) is identical across species
- Assay Duration 2.5 Hours
- Samples/Plate 39 in Duplicate
- Readout Colorimetric, 450 nm
- Sensitivity 7.38 pg/mL
- Standard Curve
Estrone-3-glucuronide (1,3,5(10)-estratrien-3-ol-17-one glucosiduronate, E1G) is the principle secreted form of circulating estradiol in mammals. Ovulation is the critical event of each menstrual cycle that occurs during the reproductive life of healthy females and the ovum can only be fertilized during the short period of time in which it is viable. The three phases of the menstrual cycle are: (i) an initial phase when there is only a low risk that would enable viable spermatozoa to survive and reach the ovum, (ii) a phase when the chance of fertilization is at a maximum, the fertile period, and (iii) a time of absolute infertility when the ovum is no Ionger viable. Clinical studies have indicated the utility of measuring estrone-3-glucuronide (E1G) and pregnanediol-3-glucuronide (PDG) in samples of urine or fecal extracts to monitor ovarian function in females.