Estrone-3-Glucuronide (E1G) ELISA Kits

Research Areas

Documentation

Specifications

• Assay Type Competitive ELISA
• Sample Types Urine, Tissue Culture Media, Fecal Extracts
• Species Estrone-3-glucuronide (E1G) is identical across species
• Assay Duration 2.5 Hours
• Samples/Plate 39 in Duplicate
• Readout Colorimetric, 450 nm
• Sensitivity 7.38 pg/mL
• Standard Curve
• Description

  Assay Principle: 

  The Estrone-3-Glucuronide (E1G) ELISA Kit quantitatively measures E1G in urine, tissue culture media, and fecal extracts. The Estrone-3-Glucuronide (E1G) ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

  Use our provided E1G standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the E1G peroxidase conjugate and the E1G polyclonal rabbit antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-E1G antibody, the E1G antigen in the sample or standard, and the E1G-peroxidase conjugate. As the E1G concentration in the sample increases, the bound E1G-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

  After the 2-hour incubation, wash away the excess E1G-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound E1G-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the E1G concentration in the samples.

  Background:

  Estrone-3-glucuronide (1,3,5(10)-estratrien-3-ol-17-one glucosiduronate, E1G) is the principal secreted form of circulating estradiol in mammals. Ovulation is the critical event of each menstrual cycle that occurs during the reproductive life of healthy females. The three phases of the menstrual cycle are:

  1. The initial phase of the menstrual cycle is where there is only a low risk that would enable viable spermatozoa to survive and reach the ovum.
  2. The fertile period is a phase when the chance of fertilization is at a maximum.
  3. A time of absolute infertility when the ovum is no longer viable. Fertilization of the ovum can only happen during the short period of viability.

  Clinical studies have indicated the utility of measuring estrone-3-glucuronide (E1G) and pregnanediol-3-glucuronide (PDG) in samples of urine or fecal extracts to monitor ovarian function in females.

• Structure