Pregnanediol-3-Glucuronide (PDG) ELISA Kit

Assay Principle:

The DetectX® Pregnanediol-3-Glucuronide (PDG) ELISA Kit accurately quantifies PDG levels in several sample types. These include extracted serum, plasma, urine, tissue culture media, and fecal extracts. This competitive ELISA kit combines precision with an efficient run time of 2.5 hours. Consult the complete kit insert for comprehensive instructions before starting the assay. The kit includes a PDG standard for creating an accurate standard curve.

Protocol Summary:

• Add standards or diluted samples into the provided transparent microtiter plate coated with goat anti-rabbit IgG antibody.
• Introduce the PDG peroxidase conjugate and PDG polyclonal rabbit antibody to each well, ensuring even mixing.
• Incubate the mixture at room temperature, shaking for 2 hours. The immunological reaction varies inversely with the PDG concentration in the sample.
• After incubation, wash away the excess conjugate and add the TMB substrate, which reacts with the bound conjugate to generate a detectable signal.
• Measure the signal intensity using a plate reader at 450nm. Calculate PDG concentration based on the standard curve.

Background:

Pregnanediol-3-Glucuronide, commonly known as PDG, is an important progesterone metabolite. Progesterone plays a vital role in the female menstrual cycle, gestation, and embryogenesis in humans and other species. As a primary progestogen, progesterone’s influence extends to the uterus, ovary, mammary gland, and brain, regulating various aspects of female reproductive health.

Progesterone also impacts other systems such as the cardiovascular, skeletal, and central nervous systems. Its neurotrophic functions in the peripheral nervous system are notable, promoting the growth and maturation of axons and aiding in the repair and renewal of myelin sheaths in regenerating nerve fibers. Measuring PDG provides insights into progesterone levels and function, offering valuable information for studies in reproductive health, hormonal balance, and the broader physiological effects of progesterone.

The DetectX® PDG ELISA Kit is an essential tool in reproductive medicine, endocrinology, and neurobiology, allowing for the precise quantification of PDG in various biological samples and aiding in the understanding of progesterone’s diverse roles in the body.