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- Assay Type Competitive ELISA
- Sample Types Extracted Serum, Extracted Plasma, Urine, Tissue CultureMedia, Fecal Extracts
- Species Pregnanediol-3-gulcuronide (PDG) is identical across all species
- Sensitivity 0.180 ng/mL
- Assay Duration 2.5 Hours
- Samples/Plate 38 in Duplicate
- Readout Colorimetric, 450 nm
- Standard Curve
The Pregnanediol-3-Glucuronide (PDG) ELISA Kit quantitatively measures PDG in extracted serum, extracted plasma, urine, tissue culture media, and fecal extracts. The Pregnanediol-3-Glucuronide (PDG) ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided PDG standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the PDG peroxidase conjugate and the PDG polyclonal rabbit antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-PDG antibody, the PDG antigen in the sample or standard, and the PDG-peroxidase conjugate. As the PDG concentration in the sample increases, the bound PDG-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 2-hour incubation, wash away the excess PDG-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound PDG-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the PDG concentration in the samples.
Pregnanediol-3-Glucuronide, also known as PDG 5β-pregnane-3α,20α-diol 3α-glucuronide, is the major metabolite of progesterone. Progesterone is a hormone involved in the female menstrual cycle, gestation, and embryogenesis of humans and other species. It belongs to a class of hormones called progestogens and is the major naturally occurring human progestogen. Progesterone is an essential regulator of human female reproductive function in the uterus, ovary, mammary gland, and brain. It also plays critical roles in the cardiovascular, skeletal, and central nervous systems. Progesterone also has neurotrophic functions in the peripheral nervous system as it activates the growth and maturation of axons and stimulates the repair and replacement of myelin sheaths in regenerating nerve fibers.