• Sample Types Cell Lysates, Tissue Extracts, Tissue Culture Media
  • Species 3',3'-Cyclic GAMP is identical across species
  • Sensitivity 0.128 pmol/mL
  • Standard Range 1,000-0.244 pmol/mL
  • Time to Answer 90 Minutes
  • Samples/Kit 39 or 231 in Duplicate
  • Stability Liquid 4°C Stable Reagents
  • Readout Colorimetric, 450 nm
  • Standard Curve
  • Description

    3’,3’-cyclic GAMP (is a key mediator of bacterial signal transduction and regulation, controlling a range of diverse targets including transcription, enzyme activity and cell cycle progression. In bacteria, 3’,3’-cGAMP signaling is regulated in-part by gene regulatory RNA elements called riboswitches that bind and respond to cGAMP with high affinity and specificity. The riboswitches  regulate  genes  involved  in  motility, biofilm formation, colonization, and virulence. The cyclic nucleotides have emerged as key players involved in bacterial physiology and inhibition studies of cGAMP signaling are ongoing as an anti-microbial strategy.
    In mammalian cells, 3’,3’-cGAMP and its eukaryotic analog 2’,3’-cGAMP produced by cGAS, bind STING (stimulator of IFN genes) and subsequently induce TBK1-IRF3-dependent production of IFN-β. Here, the cGAS-cGAMP-STING DNA sensing pathway is a key activator of the innate immune response to foreign or harmful native DNA. The cGAS-cGAMP-STING pathway plays a critical role in antiviral and antitumor immunity as well as mediating autoimmune responses. Dysregulation or aberrant activation of the pathway by self-DNA has emerged as an underlying cause of tumorigenesis and autoimmune disorders.