Oxytocin Chemiluminescent ELISA Kits

Research Areas

Documentation

Specifications

• Assay Type Competitive ELISA
• Sample Types Serum, Plasma, Saliva, Clarified Milk, Tissue Culture Media
• Sensitivity 6.33 pg/mL
• Species Oxytocin is identical across species
• Assay Duration Overnight Assay (16-18 hours)
• Samples/Plate 38 in Duplicate
• Readout Chemiluminescent
• Standard Curve
• Description

  Assay Principle: 

  The Oxytocin Chemiluminescent ELISA Kits quantitatively measure oxytocin in Serum, Plasma, Saliva, Clarified Milk, and Tissue Culture Media. The Oxytocin Chemiluminescent ELISA Kits are competitive ELISAs. Please read the complete kit insert for more information before performing this assay.

  Use our provided oxytocin standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the oxytocin peroxidase conjugate and the oxytocin polyclonal rabbit antibody. Then incubate the mixture overnight, shaking at 4C degrees. The immunological reaction occurs between the anti-oxytocin polyclonal antibody, the oxytocin antigen in the sample or standard, and the oxytocin-peroxidase conjugate. As the oxytocin concentration in the sample increases, the bound oxytocin-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

  After the overnight incubation, wash away the excess oxytocin-peroxidase and add the chemiluminescent substrate. The chemiluminescent substrate reacts with the bound corticosterone-peroxidase conjugate generating a light detected by a plate reader. Use the intensity and the standard curve to calculate the corticosterone concentration in the samples.

  Background: 

  The neuropeptides oxytocin and vasopressin were isolated and synthesized by Vincent du Vigneaud, work for which he received the Nobel Prize in Chemistry in 1955. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 &/or 8. In the oxytocin-like peptide, mesotocin; (a common peptide found in some fishes, reptiles, amphibians, marsupials, and non-mammalian tetrapods), there is isoleucine at residue eight instead of leucine. 

  Oxytocin is a neurohypophysial peptide produced in the hypothalamus. It binds to specific cell surface receptors, initiating a secondary intracellular response cascade via a phosphoinositide signaling pathway. Oxytocin is also commonly known as the “love hormone” because it plays essential roles in many reproduction-related functions in both males and females. Oxytocin functions in behavioral actions such as orgasm, social recognition, pair bonding, anxiety, and maternal behaviors. Other classical activities of oxytocin include stimulation of uterine smooth muscle contraction during labor and milk ejection during lactation.

• Structure