Oxytocin ELISA Kits

Research Areas

Documentation

Specifications

• Assay Type Competitive ELISA
• Sample Types Serum, Plasma (EDTA and Heparin), Saliva, Clarified Milk, Tissue Culture Media
• Sensitivity 17.0 pg/mL
• Species Oxytocin is identical across species
• Assay Duration Overnight Assay (16-18 hours)
• Samples/Plate 38 in Duplicate
• Readout Colorimetric, 450 nm
• Standard Curve
• Description

  Assay Principle: 

  The Oxytocin ELISA Kits quantitatively measures oxytocin in Serum, Plasma (EDTA and Heparin), Saliva, Clarified Milk, and Tissue Culture Media. The Oxytocin ELISA Kits is a competitive ELISA. Please read the complete kit insert for more information before performing this assay.

  Use our provided oxytocin standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the oxytocin peroxidase conjugate and the oxytocin polyclonal rabbit antibody. Then incubate the mixture overnight, shaking at 4C degrees. The immunological reaction occurs between the anti-oxytocin polyclonal antibody, the oxytocin antigen in the sample or standard, and the oxytocin-peroxidase conjugate. As the oxytocin concentration in the sample increases, the bound oxytocin-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

  After the overnight incubation, wash away the excess oxytocin-peroxidase and add the TMB substrate. The TMB substrate reacts with the bound oxytocin-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the oxytocin concentration in the samples.

  Background: 

  Oxytocin is a neurohypophysial peptide produced in the hypothalamus. It binds to specific cell surface receptors, initiating a secondary intracellular response cascade via a phosphoinositide signaling pathway. Oxytocin is also commonly known as the “love hormone” because it plays essential roles in many reproduction-related functions in both males and females. Oxytocin modulates behavioral actions, such as orgasm, social recognition, pair bonding, anxiety, and maternal behaviors. Other classical activities of oxytocin include stimulation of uterine smooth muscle contraction during labor and milk ejection during lactation.

  Vincent du Vigneaud first isolated and synthesized the neuropeptides oxytocin and vasopressin, earning him the Nobel Prize in Chemistry in 1955. The molecule comprises nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. Oxytocin is highly conserved across species boundaries. In the oxytocin-like peptide, mesotocin; (a common peptide found in some fishes, reptiles, amphibians, marsupials, and non-mammalian tetrapods), there is isoleucine at residue eight instead of leucine.

• Structure