• Assay Type Competitive ELISA
  • Sample Types Serum, Plasma (EDTA and Heparin), Saliva, Clarified Milk, Tissue Culture Media
  • Sensitivity 17.0 pg/mL
  • Species Oxytocin is identical across species
  • Assay Duration Overnight Assay (16-18 hours)
  • Samples/Plate 38 in Duplicate
  • Readout Colorimetric, 450 nm
  • Standard Curve
  • Description

    The neuropeptides oxytocin and vasopressin were isolated and synthesized by Vincent du Vigneaud, work for which he received the Nobel Prize in Chemistry in 1955. Oxytocin is a neurohypophysial peptide produced in the hypothalamus. The molecule consists of nine amino acids linked with a [1-6] disulfide bond and a semi-flexible carboxyamidated tail. Highly conserved across species boundaries, oxytocin-like neurohypophysial peptides are substituted primarily at residues 4 and/or 8. In the oxytocin-like peptide, mesotocin; a common peptide found in some fishes, reptiles, amphibians, marsupials and non-mammalian tetrapods, the leucine at residue 8 is substituted for isoleucine. Oxytocin binds to specific cell surface receptors, which in turn initiate a secondary intracelluar response cascade via a phosphoinositide signaling pathway. (K048)

  • Structure