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- Assay Type Competitive ELISA
- Sample Types Fecal Extracts, Urine, Tissue Culture Media
- Sensitivity 51.2 pg/mL
- Species Progesterone is identical across all species
- Assay Duration 1.5 Hours
- Samples/Plate 40 in Duplicate
- Readout Colorimetric, 450 nm
- Standard Curve
The Progesterone Metabolites ELISA Kit quantitatively measures Progesterone Metabolites in fecal extracts, urine, and tissue culture media. The Progesterone Metabolites ELISA Kit is a competitive ELISA with a run time of 1.5 hours. Please read the complete kit insert for more information before performing this assay.
Use our provided progesterone standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-rabbit IgG antibody. Add the progesterone peroxidase conjugate and the progesterone metabolites polyclonal rabbit antibody. Then incubate the mixture covered at room temperature, shaking for 1 hour. The immunological reaction occurs between the anti-progesterone metabolites antibody, the progesterone metabolites antigen in the sample or standard, and the progesterone-peroxidase conjugate. As the progesterone metabolites concentration in the sample increases, the bound progesterone-peroxidase conjugate decreases, causing a decrease in signal and vice versa.
After the 1-hour incubation, wash away the excess progesterone-peroxidase conjugate and add the TMB substrate. The TMB substrate reacts with the bound progesterone-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the progesterone metabolites concentration in the samples.
Progesterone (P4, pregn-4-ene-3,20-dione) belongs to a class of hormones called progestogens and is an essential regulator of female reproductive function in the uterus, ovary, mammary gland, and brain. Progestogens are the primary hormones in the female menstrual cycle, gestation, and embryogenesis of humans and most other species. Progesterone can be metabolized and excreted as a variety of general progesterone by-products. Common metabolites include 5-reduced progesterone (pregnane), pregnanolones, and hydroxyprogesterone. Measuring progesterone metabolites provides vital data about the reproductive status and is essential for studying the reproductive and survival strategies of wildlife and endangered species. Likewise, assays measuring progesterone metabolites give a clearer picture of hormonal regulation and reproduction.