PGE₂ Multi-Format ELISA Kits

Research Areas

Documentation

Specifications

• Assay Type Competitive ELISA
• Sample Types Serum, Plasma (EDTA and Heparin), Saliva, Urine, Tissue Culture Media
• Sensitivity 3.07 pg/mL
• Species PGE2 is identical across species
• Assay Duration 2.5 Hours or Overnight
• Samples/Plate 38 in Duplicate
• Readout Colorimetric, 450 nm
• Standard Curve
• Description

  Assay Principle: 

  The PGE2 Multi-Format ELISA Kit quantitatively measures PGE2 in serum, plasma (EDTA and Heparin), saliva, urine, and tissue culture media. The PGE2 Multi-Format ELISA Kit is a competitive ELISA with a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay.

  Use our provided PGE2 standard to generate a standard curve for the assay. Pipette the standards or diluted samples into a transparent microtiter plate coated with our goat anti-mouse IgG antibody. Add the Estradiol peroxidase conjugate and the Estradiol monoclonal mouse antibody. Then incubate the mixture covered at room temperature, shaking for 2 hours. The immunological reaction occurs between the anti-PGE2 monoclonal antibody, the PGE2 antigen in the sample or standard, and the PGE2-peroxidase conjugate. As the PGE2 concentration in the sample increases, the bound PGE2-peroxidase conjugate decreases, causing a decrease in signal and vice versa. 

  After the 2-hour incubation, wash away the excess PGE2-peroxidase and add the TMB substrate. The TMB substrate reacts with the bound PGE2-peroxidase conjugate generating a signal detected by a plate reader at 450nm. Use the intensity and the standard curve to calculate the PGE2 concentration in the samples.

  Background:

  Eicosanoid signal transduction pathways are highly conserved and involved in several physiological processes. Prostaglandins are synthesized from arachidonic acid by cyclooxygenase (COX)-1 or -2, which converts the acid into PGH2. Cytosolic or microsomal prostaglandin synthases further process PGH2 to become PGE2 or several other prostanoids. Prostacyclin is the primary cyclooxygenase product in blood vessel walls, and it is present in inflammatory fluids in similar concentrations to PGE2. Prostacyclin is a potent vasodilator and is more powerful than PGE2 in producing hyperalgesia. Various tissues have PGE2 and several pathological conditions, including inflammation, arthritis, fever, tissue injury, endometriosis, and multiple cancers. Other biological actions of PGE2 include; vasodilation, modulation of sleep/wake cycles, and facilitation of human immunodeficiency virus replication. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It is a regulator of sodium excretion and renal hemodynamics.

• Structure