Specifications

  • Catalog Number K051-H
  • Assay Type Competitive ELISA
  • Sample Types Serum, Plasma (EDTA and Heparin), Saliva, Urine, Tissue Culture Media
  • Sensitivity 3.07 pg/mL
  • Species PGE2 is identical across species
  • Assay Duration 2.5 Hours or Overnight
  • Samples/Plate 38 in Duplicate
  • Readout Colorimetric, 450 nm

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Assay Principle: 

The DetectX® PGE2 Multi-Format ELISA Kit quantitatively measures Prostaglandin E2 (PGE2) in serum, plasma (EDTA and Heparin), saliva, urine, and tissue culture media. This competitive ELISA has a run time of 2.5 hours. Please read the complete kit insert for more information before performing this assay. Use our provided PGE2 standard to generate a standard curve for the assay.

Protocol Summary:

  • Introduce standards or diluted samples into the included transparent microtiter plate coated with goat anti-mouse IgG antibody.
  • Add PGE2 peroxidase conjugate and Estradiol monoclonal mouse antibody to each well, ensuring thorough mixing.
  • Incubate the mixture at room temperature with shaking for 2 hours. The immunological reaction varies inversely with the PGE2 concentration in the sample.
  • Post-incubation, remove the excess conjugate and add the TMB substrate, which reacts with the bound conjugate to produce a detectable signal at 450 nm.
  • Calculate PGE2 concentration using a plate reader based on the standard curve.

Background:

Prostaglandin E2 (PGE2), a critical member of the prostaglandin family, plays a vital role in various physiological and pathological processes. Prostaglandins are synthesized from arachidonic acid via the cyclooxygenase (COX) enzymes. COX-1 and COX-2 convert arachidonic acid into PGH2, which is then processed into PGE2 and other prostanoids.

PGE2 is known for its involvement in inflammatory responses and is present in various tissues and pathological conditions such as arthritis, fever, tissue injury, endometriosis, and multiple cancers. Its biological actions include vasodilation, modulation of sleep/wake cycles, stimulation of bone resorption, and thermoregulatory effects. PGE2 is also significant in renal physiology, impacting sodium excretion and renal hemodynamics.

The DetectX® PGE2 Multi-Format ELISA Kit provides researchers and clinicians a reliable tool for accurate measurement of PGE2 levels in a wide range of biological samples, aiding in the study of inflammatory diseases, pain management, and other conditions where PGE2 plays a major role.

PGE2 Multi-Format ELISA Kits

$456.00$1,823.00

In Stock

One kit for rapid, sensitive, low sample volumes

The DetectX® Prostaglandin E2 (PGE2) Multi-Format ELISA Kits quantitatively measure PGE2 present in serum, plasma, urine, tissues, saliva and culture media samples.

 

SKU: K051-H Category:

Research Areas